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Phospho-Erk1/2 Pathway Antibody Sampler Kit
Phospho-Erk1/2 Pathway Antibody Sampler Kit
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Phospho-Erk1/2 Pathway Antibody Sampler Kit

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    产品介绍
    产品信息
    抗原名称
    Erk12 Pathway
    来源纯化
    Polyclonal and monoclonal antibodies are produced by immunizing rabbits with synthetic phosphopeptides corresponding to residues surrounding Ser338 of human Raf, Ser217/221 of human MEK1/2, Thr202/Tyr204 of human p44 MAP kinase, Ser380 of human p90RSK or Thr581 of human MSK1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
    简单描述
    Antibody Sampler Kit for studying ERK1 (Thr202/Tyr204) phosphate/MEK2 (Ser222/Ser226) phosphate/RSK2 (Ser386) phosphate/c-Raf (Ser338) phosphate/RSK3 (Ser377) phosphate/ERK2 (Thr185/Tyr187) phosphate/RSK1 (Ser380) phosphate/MEK1 (Ser218/Ser222) phosphate/MSK1 (Thr581) phosphate in the research area.
    研究领域
    癌症,细胞生物学,神经科学
    应用
    目标/特异性

    Specificity/Sensitivity

    Each antibody in the Phospho-Erk1/2 Pathway Antibody Sampler Kit recognizes only the phosphorylated form of its specific target.

    背景
    背景
    Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059. 1.Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 2.Baccarini, M. (2005) FEBS Lett 579, 3271-7. 3.Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. 4.Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 5.Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74. 6.Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. 7.Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 8.Marais, R. et al. (1993) Cell 73, 381-93. 9.Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. 10.Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
    研究领域
    癌症,细胞生物学,神经科学
    翻译后修饰
    Phosphate
    数据库链接
    Entrez-Gene ID
    5595,5605,6197,5894,6196,5594,6195,5604,9252
    UniProt ID
    P27361,P36507,P51812,P04049,Q15349,P28482,Q15418,Q02750,O75582

    参考图片

    Western blot analysis of extracts from NIH/3T3, HeLa and COS cells, untreated or treated with TPA, using Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427.

    Western blot analysis of extracts from 293 cells, transfected with wt MSK1 or mutant MSK1, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Thr581) Antibody.

    Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (upper), or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

    Western blot analysis of extracts from 293 cells untreated or treated with UV and HeLa cells untreated or treated with calyculin A, using Phospho-MSK1 (Thr581) Antibody.

    Immunohistochemical analysis using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb on SignalSlide™ Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103 (paraffin-embedded NIH/3T3 cells, treated with U0126 #9903 (left) or TPA #4174 (right).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb.

    Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb.

    Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 (upper), or MEK1/2 Antibody #9122 (lower).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-MSK1 (Thr581) Antibody.

    Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.

    Confocal immunofluorescent analysis of C2C12 cells, U0126-treated (left, #9903 10 μM for 1 h) or TPA-treated (right, #9905 200 nM for 15 min), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.

    Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).

    Western blot analysis of extracts from 293 cells untreated or treated with UV and HeLa cells untreated or treated with calyculin A using Phospho-MSK1 (Thr581) Antibody #9595.

    Western blot analysis of extracts from NIH3T3, HeLa and COS cells, untreated or treated with TPA, using Phospho-c-Raf (Ser338) (56A6) Rabbit mAb.

    Western blot analysis of extracts from untreated or TPA-treated 293 and NIH/3T3 cells using Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335.

    Western blot analysis of extracts from COS cells, untreated or treated with either U0126 #9903 (10 µM for 1h) or TPA #4174 (200 nM for 10 m), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107.

    Confocal immunofluorescent analysis of Drosophila egg chambers, untreated (top) or λ phosphatase-treated (bottom), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; center), or treated with TPA followed by λ phosphatase (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Western blot analysis of extracts from various cell lines, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min), Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), or Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (100 ng/mL, 15 min) as indicated, using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

    Western blot analysis of extracts from 293 cells, untreated, UV-treated or calyculin A-treated, using Phospho-MSK1 (Thr581) Antibody.

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with TPA #4174 (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

    Confocal immunofluorescent analysis of HT1080 cells, starved overnight then treated with U0126 #9903 (10 uM, 2 h; left) or PDBu (Phorbol 12,13-Dibutyrate) #12808 (100 nM, 15 m; right) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (upper), or MEK1/2 Antibody #9122 (lower).

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    货号：
    9911T
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