Phospho-Erk5 (Thr218/Tyr220) Antibody

Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2). 1.Zhou, G. et al. (1995) J Biol Chem 270, 12665-9. 2.Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8. 3.Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60. 4.Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6. 5.Kato, Y. et al. (1998) Nature 395, 713-6. 6.Kato, Y. et al. (1997) EMBO J 16, 7054-66. 7.Dong, F. et al. (2001) J Biol Chem 276, 10811-6. 8.Obara, Y. et al. (2009) J Biol Chem 284, 23564-73. 9.Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83. 10.Spiering, D. et al. (2009) J Biol Chem 284, 24972-80. 11.Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61. 12.Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.