Mouse Monoclonal Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45)

Unconjugated

Protein A or G purified

Mouse

Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34681) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

0.2 mg/ml

0.05% Sodium Azide

Monoclonal

MLANA

Immunohistochemistry Immunohistochemistry-Frozen 0.5-1ug/mlImmunohistochemistry-Paraffin 1-2 ug/mlWestern Blot 2-4 ug/ml

HuSpecies Glossary

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.