Mouse Monoclonal MITF Antibody (MITF/915) - Azide and BSA Free

Azide and BSA Free

Protein A or G purified

Mouse

Recombinant full-length human MITF protein (Uniprot: O75030)

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45161). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

1 mg/ml

No Preservative

Monoclonal

MITF

Immunofluorescence 0.5 - 1.0 ug/mlImmunohistochemistry Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml

Hu, Ca, Mu(-), Rt(-)Species Glossary

MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This monoclonal antibody recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.