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PE Mouse Anti-Human CD20(2H7)

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PE Mouse Anti-Human CD20(2H7)

品牌： BD Pharmingen

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参数

反应种属：

Rhesus, Cynomolgus, Baboon (QC Testing)

来源宿主：

Mouse C57BL/6 IgG2b, κ

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产品介绍

产品信息

耦联标记

抗原名称

CD20

宿主

Mouse C57BL/6 IgG2b, κ

免疫原

Human 6.16c1.3 B cell line

简单描述

Clone 2H7 reacts with the human form of a phosphoprotein, CD20, found on circulating peripheral blood B lymphocytes. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes nor granulocytes, of baboon and both rhesus and cynomolgus macaque monkeys. The distrubution on lymphocytes is similar to that seen with normal human donor lymphocytes, namely bright staining on B lymphocytes and weak reactivity on a small subset of CD3-positive T lymphocytes.

商品描述

同种型

Mouse C57BL/6 IgG2b, κ

克隆号

2H7

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Flow cytometry (Routinely Tested) Immunohistochemistry-frozen (Tested During Development)

推荐用量

20 µl

反应种属

Rhesus, Cynomolgus, Baboon (QC Testing)

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(3) 1. Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993; 14(2):193-204. (Biology). 2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

数据库链接

Entrez-Gene ID

931

参考图片

Profile of anti-CD20 reactivity on peripheral blood lymphocytes of Rhesus macaque (Macaca mulatta) analyzed by flow cytometry.

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货号：

556633

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