Human MSC Analysis Kit

C5

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues.  MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes.  MSCs also have immunomodulatory effects in vivo and in vitro.  In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow.  Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR.  MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  In this kit we include the panel that was proposed by the ISCT.  In addition, we designed this kit to be modular so that additional markers could be "dropped-in".  Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates.  Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples.  Individual positive markers are included for compensation set-up. Kit Components Component          Description                                                                         Size                   Vol. Per Test          Storage Buffer 51-9007661          PE hMSC Negative Cocktail                                              50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007662          PE hMSC Isotype Control Negative Cocktail          50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007663          hMSC Positive Cocktail                                                       50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007664          hMSC Isotype Control Positive Cocktail                   50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007657          FITC Mouse Anti-Human CD90                                     50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007656          PE Mouse Anti-Human CD44                                     100 Test                   5 µl                   Aqueous buffered solution containing BSA,                                                                                                                                                                   protein stabilizer, and ≤0.09% sodium azide 51-9007655          PE Mouse IgG2b, k Isotype Control                            50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007649          APC Mouse Anti-Human CD73                                     50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007648          PerCP-Cy™5.5 Mouse Anti-Human CD105                   50 Test                   5 µl                   Aqueous buffered solution containing                            (Endoglin)                                                                                                                      BSA and ≤0.09% sodium azide Description of Kit Components Vial                                                                                           Contents                                                                Purpose          hMSC Positive Cocktail                                                       CD90 FITC (Clone: 5E10)                            Cocktail to positively identify hMSCs                                                                                           CD105 PerCP-Cy5.5 (Clone: 266)                                                                                           CD73 APC (Clone: AD2) hMSC Positive Isotype Control Cocktail                   mIgG1, κ FITC (Clone: X40)                            Corresponding Isotype Control for hMSC Positive                                                                                           mIgG1, κ PerCP-Cy5.5 (Clone: X40)          Cocktail                                                                                           mIgG1, κ APC (Clone: X40) PE hMSC Negative Cocktail                                              CD34 PE (Clone:581)                                     Cocktail to identify potential contaminants                                                                                           CD11b PE (Clone: ICRF44)                                                                                           CD19 PE (Clone: HIB19)                                                                                           CD45 PE (Clone: HI30)                                                                                           HLA-DR PE (Clone: G46-6) PE hMSC Negative Isotype Control Cocktail          mIgG1, κ PE (Clone: X40)                            Corresponding isotype control for PE hMSC                                                                                           mIgG2a, κ PE (Clone:G155-178)                   Negative Cocktail FITC Mouse Anti-human CD90                                     CD90 FITC (Clone: 5E10)                            Compensation control PE Mouse Anti-Human CD44                                     CD44 PE          (Clone: G44-26)                            Compensation control/MSC positive drop-in PerCP-Cy™5.5 Mouse Anti-Human CD105                   CD105 PerCP-Cy™5.5 (Clone: 266)          Compensation control APC Mouse Anti-Human CD73                                     CD73 APC (Clone:AD2)                            Compensation control PE Mouse IgG2b, κ Isotype Control                            mIgG2b, κ (Clone: 27-35)                            Corresponding Isotype Control for PE Mouse                                                                                                                                                                   Anti-Human CD44, when used as a drop in

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues.  MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes.  MSCs also have immunomodulatory effects in vivo and in vitro.  In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow.  Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR.  MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  In this kit we include the panel that was proposed by the ISCT.  In addition, we designed this kit to be modular so that additional markers could be "dropped-in".  Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates.  Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples.  Individual positive markers are included for compensation set-up. Kit Components Component          Description                                                                         Size                   Vol. Per Test          Storage Buffer 51-9007661          PE hMSC Negative Cocktail                                              50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007662          PE hMSC Isotype Control Negative Cocktail          50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007663          hMSC Positive Cocktail                                                       50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007664          hMSC Isotype Control Positive Cocktail                   50 Test                   20 µl          Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007657          FITC Mouse Anti-Human CD90                                     50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007656          PE Mouse Anti-Human CD44                                     100 Test                   5 µl                   Aqueous buffered solution containing BSA,                                                                                                                                                                   protein stabilizer, and ≤0.09% sodium azide 51-9007655          PE Mouse IgG2b, k Isotype Control                            50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007649          APC Mouse Anti-Human CD73                                     50 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007648          PerCP-Cy™5.5 Mouse Anti-Human CD105                   50 Test                   5 µl                   Aqueous buffered solution containing                            (Endoglin)                                                                                                                      BSA and ≤0.09% sodium azide Description of Kit Components Vial                                                                                           Contents                                                                Purpose          hMSC Positive Cocktail                                                       CD90 FITC (Clone: 5E10)                            Cocktail to positively identify hMSCs                                                                                           CD105 PerCP-Cy5.5 (Clone: 266)                                                                                           CD73 APC (Clone: AD2) hMSC Positive Isotype Control Cocktail                   mIgG1, κ FITC (Clone: X40)                            Corresponding Isotype Control for hMSC Positive                                                                                           mIgG1, κ PerCP-Cy5.5 (Clone: X40)          Cocktail                                                                                           mIgG1, κ APC (Clone: X40) PE hMSC Negative Cocktail                                              CD34 PE (Clone:581)                                     Cocktail to identify potential contaminants                                                                                           CD11b PE (Clone: ICRF44)                                                                                           CD19 PE (Clone: HIB19)                                                                                           CD45 PE (Clone: HI30)                                                                                           HLA-DR PE (Clone: G46-6) PE hMSC Negative Isotype Control Cocktail          mIgG1, κ PE (Clone: X40)                            Corresponding isotype control for PE hMSC                                                                                           mIgG2a, κ PE (Clone:G155-178)                   Negative Cocktail FITC Mouse Anti-human CD90                                     CD90 FITC (Clone: 5E10)                            Compensation control PE Mouse Anti-Human CD44                                     CD44 PE          (Clone: G44-26)                            Compensation control/MSC positive drop-in PerCP-Cy™5.5 Mouse Anti-Human CD105                   CD105 PerCP-Cy™5.5 (Clone: 266)          Compensation control APC Mouse Anti-Human CD73                                     CD73 APC (Clone:AD2)                            Compensation control PE Mouse IgG2b, κ Isotype Control                            mIgG2b, κ (Clone: 27-35)                            Corresponding Isotype Control for PE Mouse                                                                                                                                                                   Anti-Human CD44, when used as a drop in

Flow cytometry (Tested During Development)

Human (Tested in Development)

研发参考(5) 1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Biology: Flow cytometry). 2. Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology: Flow cytometry). 3. Horwitz EM, Le Blanc K, Dominici M, et. al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy. 2005; 7(5):393-395. (Biology). 4. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-1301. (Biology: Flow cytometry). 5. Sánchez L, Gutierrez-Aranda I, Ligero G, et al. Enrichment of Human ESC-Derived Multipotent Mesenchymal Stem Cells with Immunosuppressive and Anti-Inflammatory Properties Capable to Protect Against Experimental Inflammatory Bowel Disease. Stem Cells. 2010; 29(2):251-262. (Biology: Cell differentiation).