
















Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |






Specificity/Sensitivity
物种反应性:
人, 小鼠, 大鼠, 猴






参考图片
Flow cytometric analysis of 293 cells using mTOR (7C10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
流式细胞技术分析293细胞;使用的抗体是 mTOR (7C10) Rabbit mAb兔单抗(蓝色),非特异性的阴性对照抗体(红色)。
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using mTOR (7C10) Rabbit mAb.
免疫组织化学方法检测石蜡包埋的人乳腺癌组织,展示胞质定位;使用的抗体是mTOR (7C10) Rabbit mAb兔单抗。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using mTOR (7C10) Rabbit mAb in the presence of control peptide (left) or mTOR Blocking Peptide #1072 (right).
免疫组织化学方法检测石蜡包埋的人肺癌组织,左图是对照肽段处理的,右图是mTOR Blocking Peptide #1072处理的;使用的抗体是mTOR (7C10) Rabbit mAb兔单抗 。
Western blot analysis of extracts from 293, A431, COS, C6, and C2C12 cells, using mTOR (7C10) Rabbit mAb.
Western blot检测分析293, A431, COS, C6 和C2C12 细胞提取物。使用抗体是mTOR (7C10) Rabbit mAb兔单抗。
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of mTOR siRNA.
Western blot检测Hela细胞提取物,用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)和 SignalSilence® mTOR siRNA II (+)转染;使用的抗体是mTOR (7C10) Rabbit mAb 兔单抗#2983 和 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。mTOR (7C10) Rabbit mAb兔单抗 确定mTOR 表达的沉默效率, α-Tubulin (11H10) Rabbit mAb 兔单抗用于上样量和 mTOR siRNA特异性的对照。
Confocal immunofluorescent analysis of mouse embryonic fibroblast (MEF) cells using mTOR (7C10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
激光共聚焦荧光法检测鼠胚胎成纤维细胞(MEF),使用的抗体是mTOR (7C10) Rabbit mAb (绿色)。微丝用DY-554 phalloidin标记(红色)。蓝色伪彩是 DRAQ5® #4084 (fluorescent DNA dye)。
Immunohistochemical analysis of paraffin-embedded mouse brain using mTOR (7C10) Rabbit mAb.