首页抗体一抗流式抗体

BD Phosflow™ PE Mouse Anti-mTOR (pS2448)

1/2

BD Phosflow™ PE Mouse Anti-mTOR (pS2448)

品牌： BD Pharmingen

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Human (QC Testing), Mouse,Rat (Predicted)

来源宿主：

Mouse IgG1, κ

展开

产品介绍

产品信息

耦联标记

抗原名称

mTOR (pS2481)

宿主

Mouse IgG1, κ

免疫原

Phosphorylated Peptide

简单描述

The O21-404 monoclonal antibody recognizes the human mammalian target of rapmycin,(mTOR) that is phosphorylated at serine residue 2448, mTOR (pS2448). mTOR belongs to the phosphoinositide-3-kinase (PI3K)-related (PIKK) family of kinases. mTOR is also known as mechanistic target of rapamycin (serine/threonine kinase), FRAP, RAFT1, and RAPT1. mTOR functions as an amino acid and ATP sensor to balance nutrient availability and cell growth. When nutrients are sufficiently available, mTOR is activated by phosphorylation at serine residue 2448 through the PI3 kinase/Akt signaling pathway. Phosphorylated mTOR in turn activates the p70 S6 kinase and contributes to the inactivation 4E-BP1, an elF4E inhibitor. In this way, mTOR participates in the upregulation of cellular protein synthesis, growth, proliferation and survival. mTOR function may be abnormally regulated in tumors.

商品描述

O21-404 The O21-404 monoclonal antibody recognizes the human mammalian target of rapmycin,(mTOR) that is phosphorylated at serine residue 2448, mTOR (pS2448). mTOR belongs to the phosphoinositide-3-kinase (PI3K)-related (PIKK) family of kinases. mTOR is also known as mechanistic target of rapamycin (serine/threonine kinase), FRAP, RAFT1, and RAPT1. mTOR functions as an amino acid and ATP sensor to balance nutrient availability and cell growth. When nutrients are sufficiently available, mTOR is activated by phosphorylation at serine residue 2448 through the PI3 kinase/Akt signaling pathway. Phosphorylated mTOR in turn activates the p70 S6 kinase and contributes to the inactivation 4E-BP1, an elF4E inhibitor. In this way, mTOR participates in the upregulation of cellular protein synthesis, growth, proliferation and survival. mTOR function may be abnormally regulated in tumors.

同种型

Mouse IgG1, κ

克隆号

克隆 O21-404 (RUO)

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing), Mouse,Rat (Predicted)

目标/特异性

mTOR (pS2481)

背景

别名

Mammalian target of rapamycin; FRAP1; RAFT1; RAPT1

翻译后修饰

Phosphate

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(6) 1. Brown EJ, Albers MW, Shin TB, et al. A mammalian protein targeted by G1-arresting rapamycin-receptor complex. Nature. 1994; 369(6483):756-758. (Biology). 2. Chiu MI, Katz H, Berlin V. RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex. Proc Natl Acad Sci U S A. 1994; 91(26):12574-12578. (Biology). 3. Holz MK, Blenis J. Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase. J Biol Chem. 2005; 280(28):26089-26093. (Biology). 4. Johnson SC, Rabinovitch PS, Kaeberlein M. mTOR is a key modulator of ageing and age-related disease. Nature. 2013; 493(7432):338-345. (Biology). 5. Nave BT, Ouwens M, Withers DJ, Alessi DR, Shepherd PR. Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem J. 1999; 344:427-431. (Biology). 6. Sengupta S, Peterson TR, Sabatini DM. Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress. Mol Cell. 2010; 40(2):310-322. (Biology).

参考图片

Analyses of mTOR (pS2448) expression in stimulated human peripheral blood B cells. Left Panel: Flow cytometric analysis of mTOR (pS2448) expression. Human B lymphocytes were prepared from peripheral blood mononuclear cells (PBMC) by negative selection using the BD IMag™ Human B Lymphocyte Enrichment Set - DM (Cat. No. 558007). The B cells were serum starved overnight in culture and were either not stimulated (dashed line histogram) or were stimulated with Type C CpG oligonucleotide (1 μM, 4 hrs; InvivoGen, Cat. No. TLRL-2395). The cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655; 10 minutes at 37°C) and then permeabilized by adding BD Phosflow™ Perm Buffer III (Cat. No. 558050; 30 minutes on ice). The cells were washed twice with BD Pharmingen™ Stain Buffer (Cat. No. 554656) and then stained with BD Phosflow™ PE Mouse Anti-mTOR (pS2448) (Cat. No. 563489) and Alexa Fluor® 647 Mouse Anti-Human CD20 (Cat. No. 558054) antibodies using the BD Biosciences Protocol for Intracellular Staining. The fluorescence histograms were derived from CD20 positive-gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer. Right Panel: Western blot analysis of mTOR (pS2448) expression. Aliquots of the unstimulated (lane 1) or CPG-stimulated (lane 2) B cells described above were made into lysates. The lysates were electrophoresed, transferred to membranes and blotted using Purified Mouse Anti-mTOR (pS2448) antibody (Clone O21-404; 2 μg/mL). Phosphorylated mTOR (pS2448) was identified as ~240-245 kDa band.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

563489

一键复制

询价

供应商现货

50Tst

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价