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Myc-Tag (9B11) Mouse mAb
Myc-Tag (9B11) Mouse mAb
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Myc-Tag (9B11) Mouse mAb

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    反应种属：
    All
    All
    来源宿主：
    Mouse
    Mouse
    产品介绍
    产品信息
    抗原名称
    Myc-Tag
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
    宿主
    Mouse
    简单描述
    Discover our reliable mouse anti Myc antibody for effective protein detection. Find out more and enhance your research today.
    商品描述

    Product Usage Information

    For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:250
    Immunohistochemistry (Paraffin)1:1500 - 1:6000
    Immunofluorescence (Immunocytochemistry)1:1000 - 1:2000
    Flow Cytometry (Fixed/Permeabilized)1:500 - 1:2000
    Chromatin IP1:50
    应用
    反应种属
    All
    目标/特异性

    Specificity/Sensitivity

    Myc-Tag (9B11) Mouse mAb detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.

    Species Reactivity:

    All Species Expected

    敏感性
    Transfected Only
    背景
    背景
    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1). 1.Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #58730.

    参考图片

    Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.Western blot fenix细胞提取物羧基末端表达Myc-tagged 蛋白（泳道1），氨基末端表达Myc-tagged 蛋白（泳道2）和对照组提取物（泳道3），所用抗体为Myc-Tag (9B11) Mouse mAb。

    Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.免疫沉淀分析细胞提取物（泳道1），提取物羧基末端表达Myc-tagged 蛋白（泳道2）或氨基末端表达Myc-tagged 蛋白。所用抗体为 Myc-Tag (9B11) Mouse mAb。

    Flow cytometric analysis of COS cells, untreated (green) or Myc transfected (blue), using Myc-Tag (9B11) Mouse mAb.流式细胞术分选COS细胞，绿色为未转染组，蓝色为Myc转染的细胞，所用抗体为using Myc-Tag (9B11) Mouse mAb。

    Immunohistochemical analysis of paraffin-embedded COS cell pellets, control (left) or transfected with a carboxy-terminal Myc tagged protein (right), using Myc-Tag (9B11) Mouse mAb.免疫组化分析石蜡包埋的COS细胞小球，左图为对照组，右图为羧基末端带有Myc标签蛋白的转染组（右图），所用抗体为Myc-Tag (9B11) Mouse mAb。

    Confocal immunofluorescent analysis of Cos cells transfected with a Myc-tagged protein (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    293T cells were either untransfected (left panel) or transfected with an Myc-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of Myc-Tag (9B11) Mouse mAb #2276, 5µl of Stat3α (D1A5) XP® Rabbit mAb #8768, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    2276S
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