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GFP (D5.1) Rabbit mAb

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GFP (D5.1) Rabbit mAb

品牌： CST

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产品介绍

产品信息

抗原名称

GFP

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying GFP jellyfish. Cited in 706 publications. Validated for WB, WB, IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, GFP (D5.1) Rabbit Monoclonal Antibody (CST #2956) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunohistochemistry (Paraffin)	1:100 - 1:400

分子量

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

GFP (D5.1) Rabbit mAb detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

Species Reactivity:

All Species Expected

敏感性

Transfected Only

背景

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1). 1.Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #34664.

数据库链接

UniProt ID

P42212

参考图片

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP (D5.1) XP® Rabbit mAb.Weatern blot分析HCC827细胞提取物，对照组和采用GFP转染组,所用抗体为GFP (D5.1) XP ® Rabbit mAb。

Flow cytometric analysis of HCC827 cells, untransfected (blue) or GFP-transfected (green), using GFP (D5.1) XP® Rabbit mAb.流式细胞术分析HCC827细胞，蓝色为未转染组，绿色为GFP转染组，所用抗体为GFP (D5.1) XP ® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or GFP-transfected (right), using GFP (D5.1) XP® Rabbit mAb.免疫组化分析石蜡包埋的HCC827细胞，上图为未处理组，下图为GFP转染组，所用抗体为GFP (D5.1) XP ® Rabbit mAb。

Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein (green) using GFP (D5.1) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析表达GFP标签融合蛋白的COS-7细胞，所用抗体为GFP (D5.1) XP ® Rabbit mAb (红色)，蓝色伪彩为荧光染料，信息为DRAQ5 ® #4084。

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货号：

2956T

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