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Nanog (D2A3) XP ® Rabbit mAb
Nanog (D2A3) XP ® Rabbit mAb

Nanog (D2A3) XP ® Rabbit mAb

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品牌： CST
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    分子量：
    29-42
    29-42
    反应种属：
    Mouse
    Mouse
    展开
    产品介绍
    产品信息
    抗原名称
    Nanog
    来源纯化
    使用与小鼠 nanog 蛋白氨基末端周围残基相对应的合成肽，对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying Nanog mouse. Cited in 87 publications. Validated for WB, IP, IF, F, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Nanog (D2A3) XP® Rabbit Monoclonal Antibody (CST #8822) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:100
    Immunofluorescence (Immunocytochemistry)1:1000
    Flow Cytometry (Fixed/Permeabilized)1:200
    Chromatin IP1:100
    Chromatin IP-seq1:100
    CUT&RUN1:100
    分子量
    29-42
    研究领域
    癌症,发育生物学与干细胞研究,神经科学
    应用
    反应种属
    Mouse
    目标/特异性

    Specificity/Sensitivity

    Nanog (D2A3) XP® Rabbit mAb 识别内源水平的小鼠 nanog 总蛋白。该抗体预计可识别 nanog 1a 和 1b 两种同工型。该抗体不会与人类 nanog 蛋白发生交叉反应。

    物种反应性：

    小鼠

    敏感性
    Endogenous
    背景
    背景
    Nanog 是含有同源结构域的转录因子，并且这种转录因子是胚胎干细胞中维持多能性和自我更新所必需的 (1)。Nanog 表达通过一系列因子控制，这些因子包括 Sox2 和关键的多能性调节蛋白 Oct-4 (1)。体细胞再编程方面的最新进展利用了转录因子组合的病毒表达，这些转录因子包括 nanog、Oct-4、Sox2、KLF4、c-Myc 和 LIN28 (2,3)。 Kim, J. et al. (2008) Cell 132, 1049-61. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9. Yu, J. et al. (2007) Science 318, 1917-20.
    研究领域
    癌症,发育生物学与干细胞研究,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    71950
    UniProt ID
    Q80Z64

    参考图片

    Western blot analysis of extracts from F9 and murine iPS cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific).

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 F9 cells and either 5 μl of Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Flow cytometric analysis of NIH/3T3 (blue) and F9 (green) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific).

    Confocal immunofluorescent analysis of F9 (left) and NIH/3T3 (right) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red).

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    货号：
    8822T
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    20ul
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