首页抗体一抗一抗

NeuN (D3S3I) Rabbit mAb

品牌： CST

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

分子量：

46-55

反应种属：

Human,Mouse,Rat,

展开

产品介绍

产品信息

抗原名称

NeuN

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NeuN protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying NeuN. Cited in 127 publications. Validated for Western Blotting, Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, NeuN (D3S3I) Rabbit Monoclonal Antibody (CST #12943) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:250 - 1:1000

分子量

46-55

研究领域

神经科学

应用

反应种属

Human,Mouse,Rat,

目标/特异性

Specificity/Sensitivity

NeuN (D3S3I) Rabbit mAb recognizes endogenous levels of total NeuN protein. A 30 kDa protein of unknown origin may be detected.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). 1.Mullen, R.J. et al. (1992) Development 116, 201-11. 2.Kim, K.K. et al. (2009) J Biol Chem 284, 31052-61. 3.Kim, K.K. et al. (2013) J Cell Biol 200, 443-58.

研究领域

神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

146713

UniProt ID

A6NFN3

参考图片

Confocal immunofluorescent analysis of normal mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D3S3I) Rabbit mAb (green), and GFAP (GA5) Mouse mAb (Alexa Fluor®555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (Fluorescent DNA dye).

共聚焦免疫荧光分析正常小鼠海马(左)，皮质（中）和小脑（右），使用的抗体是NeuN (D3S3I) Rabbit mAb 兔单抗(绿色)，和 GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (红色)。蓝色伪彩= DRAQ5® #4084 (荧光 DNA 染料)。

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

12943T

一键复制

询价

供应商现货

20ul

100ul

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价