Human Neural Cell Sorting Kit

A7

The BD Stemflow™ Neural Cell Isolation Kit was designed to allow the isolation of neural stem cells (NSCs) derived from human pluripotent stem cells or the isolation of neurons and glial cells from differentiated NSCs.  Following a variety of established neural induction protocols, NSCs are sorted from the heterogeneous cell culture utilizing the included antibody conjugates to five cell surface markers.  This method greatly reduces the amount of variability between NSC isolations as opposed to traditional methods like manual isolation.  In addition, four of the five included conjugates can be used to isolate neurons from glia from differentiated NSCs. Kit Components Component          Description                                                                                  Size                   Vol. Per Test          Storage Buffer 51-9007757          PE Mouse Anti-Human CD24                                              20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007758          PerCP-Cy™5.5 Mouse Anti-Human CD271                            20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007759          PerCP-Cy™5.5 Mouse Anti-Human CD44                            20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007760          PE-Cy™7 Mouse Anti-Human CD15                                     20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007761          APC Mouse Anti-Human CD184                                              20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007762          PE Mouse IgG1, κ Isotype Control                                     10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007763          PerCP-Cy™5.5 Mouse IgG1, κ                                              10 Test                   5 µl                   Aqueous buffered solution containing                            Isotype Control for 51-007758                                                                                           BSA and <0.09% sodium azide 51-9007764          PerCP-Cy™5.5 Mouse IgG1, κ                                              10 Test                   5 µl                   Aqueous buffered solution containing                            Isotype Control for 51-9007759                                                                                           BSA and <0.09% sodium azide 51-9007765          PE-Cy™7 Mouse IgM, κ Isotype Control                            10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007766          APC Mouse IgG1, κ Isotype Control                                     10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007767          Negative Control CompBead Plus                                              1.5 ml                   1 drop          Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007768          Anti-Mouse Ig, κ CompBead Plus                                              1.5 ml                   1 drop          Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide Specificity          Clone                   Molecule                            NSC Phenotype                   Neuron Phenotype                   Glial Phenotype CD15                   HI98                   X-hapten, SSEA-1                       -/+                                     Low                                     NA CD24                   ML5                   Heat Stable Antigen               +                                              +                                              NA CD44                   G44-26                   H-CAM                                           -                                              -                                              + CD184                   12G5                   CXCR4, Fusin                                 +                                              -                                              + CD271                   C40-1457          NGF-Receptor                                  -                                              NA                                              NA

The BD Stemflow™ Neural Cell Isolation Kit was designed to allow the isolation of neural stem cells (NSCs) derived from human pluripotent stem cells or the isolation of neurons and glial cells from differentiated NSCs.  Following a variety of established neural induction protocols, NSCs are sorted from the heterogeneous cell culture utilizing the included antibody conjugates to five cell surface markers.  This method greatly reduces the amount of variability between NSC isolations as opposed to traditional methods like manual isolation.  In addition, four of the five included conjugates can be used to isolate neurons from glia from differentiated NSCs. Kit Components Component          Description                                                                                  Size                   Vol. Per Test          Storage Buffer 51-9007757          PE Mouse Anti-Human CD24                                              20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007758          PerCP-Cy™5.5 Mouse Anti-Human CD271                            20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007759          PerCP-Cy™5.5 Mouse Anti-Human CD44                            20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007760          PE-Cy™7 Mouse Anti-Human CD15                                     20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007761          APC Mouse Anti-Human CD184                                              20 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007762          PE Mouse IgG1, κ Isotype Control                                     10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007763          PerCP-Cy™5.5 Mouse IgG1, κ                                              10 Test                   5 µl                   Aqueous buffered solution containing                            Isotype Control for 51-007758                                                                                           BSA and <0.09% sodium azide 51-9007764          PerCP-Cy™5.5 Mouse IgG1, κ                                              10 Test                   5 µl                   Aqueous buffered solution containing                            Isotype Control for 51-9007759                                                                                           BSA and <0.09% sodium azide 51-9007765          PE-Cy™7 Mouse IgM, κ Isotype Control                            10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007766          APC Mouse IgG1, κ Isotype Control                                     10 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007767          Negative Control CompBead Plus                                              1.5 ml                   1 drop          Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide 51-9007768          Anti-Mouse Ig, κ CompBead Plus                                              1.5 ml                   1 drop          Aqueous buffered solution containing                                                                                                                                                                            BSA and <0.09% sodium azide Specificity          Clone                   Molecule                            NSC Phenotype                   Neuron Phenotype                   Glial Phenotype CD15                   HI98                   X-hapten, SSEA-1                       -/+                                     Low                                     NA CD24                   ML5                   Heat Stable Antigen               +                                              +                                              NA CD44                   G44-26                   H-CAM                                           -                                              -                                              + CD184                   12G5                   CXCR4, Fusin                                 +                                              -                                              + CD271                   C40-1457          NGF-Receptor                                  -                                              NA                                              NA

Human (Reactivity Confirmed in Development)

研发参考(2) 1. Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009; 27(3):275-280. (Biology: Cell differentiation). 2. Yuan SH, Martin J, Elia J, et al. Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells. PLoS ONE. 6(3)(Methodology: Flow cytometry).