Human Neural Lineage Analysis Kit

The BD Stemflow™ Human Neural Lineage Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the analysis of neural differentiation cultures. The antibody specificities that are provided in this kit and the neural cell populations they can identify are listed in the table below. The antibody components of this kit can be used individually or in combinations of up to four. All Antibodies in this kit have been verified by western blot and image analysis. Additional antibody formats that can enable more complex panels are available at www.bdbiosciences.com. The kit also includes BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III. Kit Components Component          Description                                                                         Size                   Vol. Per Test          Storage Buffer 51-9007227          PerCP-Cy™5.5 Mouse Anti-Sox2                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007228          Alexa Fluor® 647 Mouse Anti-GFAP                            25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007229          PE Mouse Anti-Doublecortin                                              25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007230          Alexa Fluor® 647 Mouse Anti-Nestin                            25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007231          Alexa Fluor® 488 Mouse Anti-Human Ki-67          25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007232          PerCP-Cy™5.5 Mouse Anti-Human Sox1                   25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007233          FITC Mouse Anti-Human CD44                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9006607          Perm Buffer III                                                                50 ml                                     51-9006276          Fixation Buffer                                                                50 ml Specificity                   Clone                            Molecule                                              Neural cell population identified CD44                            G44-26                            H-CAM                                              Glial cells, astrocytes, astrocyte precursors Ki-67                            B56                                     Nuclear proliferation marker          All proliferating cell types Doublecortin                   30/Doublecortin          Neuroflilament                            Immature post-mitotic neurons Sox2                            O30-678                            Transcription factor                            Glial cells, embryonic and neural stem cells Sox1                            N23-844                            Transcription factor                            Glial cells and neural stem cells GFAP                            1B4                                     Filament                                              Astrocytes Nestin                            25/Nestin                            Filament                                              Glial cells, astrocytes, embryonic and neural stem cells

The BD Stemflow™ Human Neural Lineage Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the analysis of neural differentiation cultures. The antibody specificities that are provided in this kit and the neural cell populations they can identify are listed in the table below. The antibody components of this kit can be used individually or in combinations of up to four. All Antibodies in this kit have been verified by western blot and image analysis. Additional antibody formats that can enable more complex panels are available at www.bdbiosciences.com. The kit also includes BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III. Kit Components Component          Description                                                                         Size                   Vol. Per Test          Storage Buffer 51-9007227          PerCP-Cy™5.5 Mouse Anti-Sox2                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007228          Alexa Fluor® 647 Mouse Anti-GFAP                            25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007229          PE Mouse Anti-Doublecortin                                              25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007230          Alexa Fluor® 647 Mouse Anti-Nestin                            25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007231          Alexa Fluor® 488 Mouse Anti-Human Ki-67          25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007232          PerCP-Cy™5.5 Mouse Anti-Human Sox1                   25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9007233          FITC Mouse Anti-Human CD44                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤ 0.09% sodium azide 51-9006607          Perm Buffer III                                                                50 ml                                     51-9006276          Fixation Buffer                                                                50 ml Specificity                   Clone                            Molecule                                              Neural cell population identified CD44                            G44-26                            H-CAM                                              Glial cells, astrocytes, astrocyte precursors Ki-67                            B56                                     Nuclear proliferation marker          All proliferating cell types Doublecortin                   30/Doublecortin          Neuroflilament                            Immature post-mitotic neurons Sox2                            O30-678                            Transcription factor                            Glial cells, embryonic and neural stem cells Sox1                            N23-844                            Transcription factor                            Glial cells and neural stem cells GFAP                            1B4                                     Filament                                              Astrocytes Nestin                            25/Nestin                            Filament                                              Glial cells, astrocytes, embryonic and neural stem cells

Flow cytometry (Routinely Tested)

Human (QC Testing)

研发参考(5) 1. Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009; 27(3):275-280. (Methodology: Cell differentiation). 2. Itsykson P, Ilouz N, Turetsky T, Goldstein RS et al. Derivation of neural precursors from human embryonic stem cells in the presence of noggin. Mol Cell Neurosci. 2005; 30(1):24-36. (Methodology: Cell differentiation). 3. Koch P, Opitz T, Steinbeck JA, Ladewig J, Brüstle O. A rosette-type, self-renewing human ES cell-derived neural stem cell with potential for in vitro instruction and synaptic integration. Proc Natl Acad Sci U S A. 2009; 106(9):3225-3230. (Biology). 4. Reubinoff BE, Itsykson P, Turetsky T, et al. Neural progenitors from human embryonic stem cells. Nat Biotechnol. 2001; 19(12):1117-1118. (Methodology: Cell differentiation). 5. Yeo GW, Xu X, Liang TY, et al. Alternative splicing events identified in human embryonic stem cells and neural progenitors. PLoS Comput Biol. 2007; 3(10):1951-1967. (Biology).