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NF-kappaB Family Member Antibody Sampler Kit
NF-kappaB Family Member Antibody Sampler Kit
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NF-kappaB Family Member Antibody Sampler Kit

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    产品介绍
    产品信息
    抗原名称
    NF-kappaB Family Member
    来源纯化
    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to amino acid residues near the carboxy terminus of human NF-κB p65, surrounding Ser424 of human RelB, surrounding Leu65 of human c-Rel protein, surrounding Gly415 of human NF-κB p105/p50 protein, surrounding Glu498 of human NF-κB p65/RelA protein, and near the amino terminus of human NF-κB2 p100/p52. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to amino acid residues at the the carboxy terminus of human NF-κB1 p105 and near the amino terminus of human NF-κB2 p100/p52. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
    简单描述
    Antibody Sampler Kit for studying in the research area.
    应用
    目标/特异性

    Specificity/Sensitivity

    Each antibody in this kit recognizes endogenous levels of its target protein regardless of post-translational modification state such as phosphorylation or acetylation. The NF-κB1 p105/p50 (D7H5M) Rabbit mAb #12540 detects both the precursor protein p105 and its cleavage product p50, while the NF-κB1 p105 Antibody #4717 only detects p105 and will not cross-react with p50. Both the NF-κB2 p100/p52 Antibody #4882 and the NF-κB2 p100/p52 (18D10) Rabbit mAb (Human Specific) #3017 will cross-react with the precursor protein p100 and its cleavage product p52.

    背景
    背景
    Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). 1.Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2.Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3.Haskill, S. et al. (1991) Cell 65, 1281-9. 4.Thompson, J.E. et al. (1995) Cell 80, 573-82. 5.Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6.Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7.Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8.Chen, Z.J. et al. (1996) Cell 84, 853-62. 9.Senftleben, U. et al. (2001) Science 293, 1495-9. 10.Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11.Xiao, G. et al. (2001) Mol Cell 7, 401-9.

    参考图片

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    Western blot analysis of extracts from THP-1, L929, C6, and COS cells, using NF-kappaB2 p100 Antibody.

    Flow cytometric analysis of HeLa cells, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific) (blue) compared to a nonspecific negative control antibody (red).

    Western blot analysis of extracts from HeLa, and COS cells, using NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific).

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific).

    Western blot analysis of extracts from K562, Raji and 293 cells, using c-Rel Antibody.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

    Immunohistochemical analysis of paraffin-embedded Hodgkin's lymphoma, showing nuclear and cytoplasmic localization using c-Rel Antibody.

    Immunohistochemical analysis of paraffin-embedded human skin, using c-Rel Antibody.

    Flow cytometric analysis of K562 cells using c-Rel Antibody (blue) compared to a nonspecific negative control antibody (red).

    Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using NF-κB1 p105 Antibody.

    Western blot analysis of extracts from Raji, THP-1 and BaF3 cells using RelB (C1E4) Rabbit mAb.

    Western blot analysis of extracts from Raji, THP-1 and BaF3 cells using RelB (C1E4) Rabbit mAb #4922.Western免疫印迹。用RelB (C1E4) Rabbit mAb #4922研究Raji, THP-1和BaF3 细胞的细胞提取液。

    Western blot analysis of extracts from K562, Raji and 293 cells using c-Rel Antibody #4727.Western免疫印迹。用 c-Rel Antibody #4727研究 K562, Raji 和293 细胞的细胞提取液。

    Western blot analysis of extracts from HeLa cells, untreated or treated with TNF-α (12 ng/ml) for the indicated amounts of time, using NF-kB p105/p50 Antibody #3035.Western免疫印迹。用 NF-kB p105/p50 Antibody #3035研究未经处理的和经TNF-α (12 ng/ml) 处理的一定时间的HeLa细胞的细胞提取液。

    Western blot analysis of extracts from various cell types using NF-kB2 p100/p52 Antibody #4882.Western免疫印迹。用NF-kB2 p100/p52 Antibody #4882研究各种细胞类型的细胞提取液。

    Western blot analysis of extracts from HeLa and COS cells using NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific) #3017.Western免疫印迹。用NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific) #3017研究 HeLa 和COS 细胞的细胞提取液。

    Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TNFα-treated (#2169, 20 ng/ml for 20 min, right), using c-Rel Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

    Western blot analysis of extracts from various cell lines using NF-κB p65 (L8F6) Mouse mAb.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (30 ng/ml, 1 hr) and either 10 μl of NF-κB p65 (L8F6) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (right), using NF-κB p65 (L8F6) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded OVCAR8 cell pellets treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (left) or treated with SignalSilence® NF-κB p65 siRNA I #6261 (right), using NF-κB p65 (L8F6) Mouse mAb.

    Immunohistochemical analysis of human chronic cholecystitis tissue using NF-κB p65 (L8F6) Mouse mAb.

    Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® NF-κB p65 siRNA I #6261 (+), using NF-κB p65 (L8F6) Mouse mAb (upper) or α-Tubulin (11Η10) Rabbit mAb #2125 (lower). The NF-κB p65 (L8F6) Mouse mAb confirms silencing of NF-κB p65 expression, while the α-Tubulin (11Η10) Rabbit mAb is used as a loading control.

    Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/mL, 20 min; right), using NF-κB p65 (L8F6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Flow cytometric analysis of HeLa cells using NF-κB p65 (L8F6) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

    Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) or treated with hTNF-α #8902 (20 ng/ml, 20 min) (right), using NF-κB p65 (D14E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with hTNF-α #8902 (30 ng/ml, 1 hr) and either 5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using NF-κβ p65 (L8F6) Mouse mAb #6956.Western免疫印迹。用NF-κβ p65 (L8F6) Mouse mAb #6956研究各种细胞系的细胞提取液。

    Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using NF-κβ1 p105 Antibody #4717.Western免疫印迹。用NF-κβ1 p105 Antibody #4717研究经TPA(#9905, 80 nM，24 h) 分化处理然后经过1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。

    Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

    Immunohistochemical analysis of paraffin-embedded human chronic cholecystitis using NF-κB p65 (D14E12) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb #8242.Western免疫印迹。用NF-κB p65 (D14E12) XP® Rabbit mAb #8242研究各种细胞系的细胞提取液。

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (30 ng/ml) for 1 hour and either 10 μl of NF-κB1 p105/p50 (D7H5M) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IAP2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Western blot analysis of extracts from various cell lines using NF-κB1 p105/p50 (D7H5M) Rabbit mAb.

    Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml) for the indicated times, using NF-κB1 p105/p50 (D7H5M) Rabbit mAb.

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    货号：
    4766T
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