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NF-kappaB p65 (D14E12) XP ® Rabbit mAb
NF-kappaB p65 (D14E12) XP ® Rabbit mAb
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NF-kappaB p65 (D14E12) XP ® Rabbit mAb

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品牌： CST
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    分子量：
    65
    65
    反应种属：
    Human,Mouse,Rat,Hamster,Monkey,Dog,
    Human,Mouse,Rat,Hamster,Monkey,Dog,
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    产品介绍
    产品信息
    抗原名称
    NF-kappaB p65
    来源纯化
    通过采用与人 NF-κB p65/RelA 蛋白的 Glu498 周围的残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying NFkB-p65. Cited in 4333 publications. Validated for WB, WB, IP, IHC, IF, F, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, NF-κB p65 (D14E12) XP® Rabbit Monoclonal Antibody (CST #8242) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:100
    Immunohistochemistry (Paraffin)1:400 - 1:1600
    Immunofluorescence (Immunocytochemistry)1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized)1:400 - 1:1600
    Chromatin IP1:100
    Chromatin IP-seq1:100
    CUT&RUN1:100
    分子量
    65
    研究领域
    免疫学和肿瘤学,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Hamster,Monkey,Dog,
    目标/特异性

    Specificity/Sensitivity

    NF-κB p65 (D14E12) XP® Rabbit mAb 可识别 NF-κB p65/RelA 总蛋白的内源水平。它不与其他 NF-κB/Rel 家族成员发生交叉反应。

    物种反应性：

    人, 小鼠, 大鼠, 仓鼠 , 猴, 犬

    敏感性
    Endogenous
    背景
    背景

    核转录因子 κB (NF-κB)/Rel 家族的转录因子，在炎症和免疫应答中发挥重要作用 (1,2)。在哺乳动物中有五个家族成员：RelA、c-Rel、RelB、NF-κB1 (p105/p50) 和 NF-κB2 (p100/p52)。p105 和 p100 都可由蛋白酶体经蛋白水解处理后，分别产生 p50 和 p52。Rel 蛋白结合 p50 和 p52 形成二聚体，二聚体再结合 DNA，调节转录活动。未刺激细胞中 NF-κB 被 IκB 抑制性蛋白阻隔在细胞浆中 (3-5)。NF-κB 活化剂可诱导 IκB 蛋白的磷酸化，并通过泛素-蛋白酶体通路将其靶标进行快速降解，释放 NF-κB 进入胞核，使其调节基因表达 (6-8)。NIK 和 IKKα (IKK1) 可调节 NF-κB2 (p100) 的磷酸化和加工以产生 p52，后者再转位入胞核 (9-11)。 Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. Haskill, S. et al. (1991) Cell 65, 1281-9. Thompson, J.E. et al. (1995) Cell 80, 573-82. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. Chen, Z.J. et al. (1996) Cell 84, 853-62. Senftleben, U. et al. (2001) Science 293, 1495-9. Coope, H.J. et al. (2002) EMBO J 21, 5375-85. Xiao, G. et al. (2001) Mol Cell 7, 401-9. Kozako, T. et al. (2015) Sci Rep 5, 11345.

    研究领域
    免疫学和肿瘤学,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #69994.
    数据库链接
    Entrez-Gene ID
    5970
    UniProt ID
    Q04206

    参考图片

    Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb.Western免疫印迹。用NF-κB p65 (D14E12) XP® Rabbit mAb研究各种类型细胞的细胞提取液。

    Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) or treated with hTNF-α #8902 (20 ng/ml, 20 min) (right), using NF-κB p65 (D14E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HT-1080 细胞核，未经处理 (左图)或 经hTNF-α #8902 (20 ng/ml, 20 min) (右图)处理, 所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb (绿色) 肌动蛋白微丝用DY-554 phalloidin标记（红色)。Blue pseudocolor=DRAQ5® #4084 DNA 荧光染料。

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with hTNF-α #8902 (30 ng/ml, 1 hr) and either 5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。HeLa细胞培养至4 x 106，并用hTNF-α #8902 (30 ng/ml, 1 hr)处理后，将染色质交联到玻片上,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb抗体或2 μl Normal Rabbit IgG #2729 。对富集的DNA做real-time PCR,所用引物为SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

    Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞仪研究HeLa细胞，所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb (蓝色) 浓度相匹配的 Rabbit (DA1E) mAb IgG XP® 同型对照#3900 (红色)。

    Immunohistochemical analysis of paraffin-embedded human chronic cholecystitis using NF-κB p65 (D14E12) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人慢性胆囊炎组织。所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb。

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    货号：
    8242S
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