Human NK Cell Enrichment Set - DM

Enrichment of NK cells from human blood. PBMC were labeled with the BD IMag™ Human NK Cell Enrichment Set - DM (Cat. No. 557987) and separated on the BD™ IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC Anti-Human CD56 (Cat. No. 555518) to detect NK cells and a mixture of PE Mouse Anti-Human CD3 (Cat. No. 555333), Anti-Human CD19 (Cat. No. 555413), Anti-Human CD36 (Cat. No. 555455), Anti-Human CD123 (Cat. No. 555644), and Anti-Human CD235a (Cat. No. 555570) monoclonal antibodies to detect non-NK leukocytes (including NK-T cells) and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step typically results in >5% increased purity with less than a 5% decrease in recovery. Far right panel shows the positive fraction.