Notch Receptor Interaction Antibody Sampler Kit

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Notch Receptor Interaction Antibody Sampler Kit

品牌： CST

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产品介绍

产品信息

抗原名称

Notch Receptor Interaction

来源纯化

Monoclonal antibodies are produced by immunizing animals either with a recombinant protein specific to the amino terminus of human TACE protein or with a synthetic peptide corresponding to residues surrounding Glu1140 (intracellular region) of human Jagged1 protein, residues surrounding Ala117 of human Jagged2 protein, residues surrounding Ala570 of human Numb protein, or residues near the carboxy terminus of human ADAM9 protein or residues surrounding Gln110 of human RBPSUH protein.

简单描述

Antibody Sampler Kit for studying ADAM9/DLL4/Jagged2/Jagged1/DLL1/RBPSUH/DLL3/TACE/Numb in the research area.

研究领域

癌症,细胞生物学,发育生物学与干细胞研究,纤维化,神经科学

应用

目标/特异性

Specificity/Sensitivity

ADAM9 (D64B5) Rabbit mAb, DLL4 Antibody, Jagged1 (28H8) Rabbit mAb, Jagged2 (C23D2) Rabbit mAb, Numb (C29G11) Rabbit mAb, RBPSUH (D10A4) XP® Rabbit mAb, and TACE (D22H4) Rabbit mAb recognize endogenous levels of total respecitive protein. DLL1 Antibody recognizes only transfected levels of DLL1 protein. It does not recognize transfected levels of rat DLL3 or human DLL4. DLL3 (G93) Antibody recognizes only transfected levels of DLL3 protein. It does not recognize transfected levels of rat DLL1 or human DLL4. Jagged1 (28H8) Rabbit mAb does not cross-react with Jagged2. Jagged2 (C23D2) Rabbit mAb does not cross-react with Jagged1.

背景

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the Delta, Serrate, Lag2 (DSL) single-pass type I membrane proteins. DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a similiar manner, suggesting bi-directional signaling through receptor-ligand interactions (4-6).TNF-α converting enzyme (TACE), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as “shedding". TACE is abundantly expressed in many adult tissues, but in fetal development, expression is differentially regulated (7). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (8).Recombining Binding Protein, SUppressor of Hairless (RBPSUH), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (9-11).Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (12,13). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (14-16). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (17). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (18,19). 1.Wilson, A. and Radtke, F. (2006) FEBS Lett 580, 2860-8. 2.Hansson, E.M. et al. (2004) Semin Cancer Biol 14, 320-8. 3.Chiba, S. (2006) Stem Cells 24, 2437-47. 4.Bland, C.E. et al. (2003) J Biol Chem 278, 13607-10. 5.Six, E. et al. (2003) Proc Natl Acad Sci U S A 100, 7638-43. 6.LaVoie, M.J. and Selkoe, D.J. (2003) J Biol Chem 278, 34427-37. 7.Black, R.A. et al. (1997) Nature 385, 729-33. 8.Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43. 9.Ehebauer, M. et al. (2006) Sci STKE 2006, cm7. 10.Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46. 11.Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33. 12.Berdnik, D. et al. (2002) Dev Cell 3, 221-31. 13.Santolini, E. et al. (2000) J Cell Biol 151, 1345-52. 14.Dho, S.E. et al. (1999) J Biol Chem 274, 33097-104. 15.Verdi, J.M. et al. (1999) Proc Natl Acad Sci U S A 96, 10472-6. 16.Verdi, J.M. et al. (1999) Proc Natl Acad Sci U S A 96, 10472-6. 17.McGill, M.A. and McGlade, C.J. (2003) J Biol Chem 278, 23196-203. 18.Verdi, J.M. et al. (1996) Curr Biol 6, 1134-45. 19.Reugels, A.M. et al. (2006) Dev Dyn 235, 934-48.

研究领域

癌症,细胞生物学,发育生物学与干细胞研究,纤维化,神经科学

翻译后修饰

Unmodified

数据库链接

Entrez-Gene ID

8754,54567,3714,182,28514,3516,10683,6868,8650

UniProt ID

Q13443,Q9NR61,Q9Y219,P78504,O00548,Q06330,Q9NYJ7,P78536,P49757

参考图片

Western blot analysis of extracts from various cell lines using Jagged2 (C23D2) Rabbit mAb #2210.

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing rat DLL1 protein, using DLL1 Antibody #2588.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of total cell lysates from HepG2 and LNCaP cells, using Jagged1 (28H8) Rabbit mAb.

Western blot analysis of total cell lysates from HeLa, SK-OV-3 and SR cells using Jagged2 (C23D2) Rabbit mAb.

Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing rat DLL3 protein, using DLL3 (G93) Antibody.

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing rat DLL1 protein, using DLL1 Antibody.

Western blot analysis of extracts from HUVEC and COS cells, untransfected or transiently transfected with a construct expressing human DLL4, using DLL4 Antibody.

Western blot analysis of extracts from various cell lines using Numb (C29G11) Rabbit mAb.

Flow cytometric analysis of A-204 cells using Numb (C29G11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Confocal immunofluorescent analysis of HeLa cells using Numb (C29G11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell types using ADAM9 (D64B5) Rabbit mAb.

Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with peptide N-glycosidase F (PNGase F), using TACE (D22H4) Rabbit mAb.

Western blot analysis of extracts from various cell lines using RBPSUH (D10A4) XP^®Rabbit mAb.

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM, 3 d) and then either harvested immediately (left panel) or washed and cultured in fresh media for 3 h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 10 µl of RBPSUH (D10A4) XP^®Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP^® Human HES4 Promoter Primers #7273, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western blot analysis of extracts from HepG2 and LNCaP cells using Jagged1 (28H8) Rabbit mAb #2620.

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing rat DLL3 protein, using DLL3 (G93) Antibody #2483.

Western blot analysis of extracts from HUVEC and COS cells, untransfected or transiently transfected with a construct expressing human DLL4, using DLL4 Antibody #2589.

Western blot analysis of extracts from various cell lines using ADAM9 (D64B5) Rabbit mAb #4151.

Western blot analysis of extracts from Raji and Jurkat cells, untreated (-) or treated with peptide N-glycosidase F (PNGase F; +), using TACE (D22H4) Rabbit mAb #6978.

Western blot analysis of extracts from various cell lines using Numb (C29G11) Rabbit mAb #2756.

Western blot analysis of extracts from various cell lines using RBPSUH (D10A4) XP® Rabbit mAb #5313.

Immunohistochemical analysis of paraffin-embedded mouse lymph node using RBPSUH (D10A4) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RBPSUH (D10A4) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded E18.5 mouse lung, Rbpjk ^F/+ Shh^+/+ (wild type, left) or Rbpjk ^F/- Shh^cre/+ (Rbpjk conditional knock out, right), using RBPSUH (D10A4) XP^® Rabbit mAb. Note lack of staining in the bronchial epithelial cells in the conditional knock out tissue (right). Tissue courtesy of Dr. Wellington Cardosa, Boston University School of Medicine.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RBPSUH (D10A4) XP^® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using RBPSUH (D10A4) XP^® Rabbit mAb.

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货号：

8658T

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