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1:500-1:2000IF
1:200-1:1000

p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer.


12 months from date of receipt / reconstitution, -20 °C as supplied
参考图片
ICC shows positive staining in HeLa cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control:ICC shows negative staining in MCF7 cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded Human cervical squamous cell carcinoma. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.