p16 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-303-206)
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p16 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-303-206)

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    反应种属:
    Hu
    Hu
    来源宿主:
    Rabbit
    Rabbit
    展开
    产品介绍
    产品信息
    耦联标记
    Alexa Fluor® 555
    纯化方式
    Protein A
    抗原名称
    p16
    宿主
    Rabbit
    免疫原
    Recombinant Protein
    同种型
    IgG
    克隆号
    SDT-303-206
    浓度
    2 mg/ml
    性状
    Liquid
    缓冲体系
    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
    产品类型
    Recombinant mAb
    应用
    实验应用
    ICC、IF
    反应种属
    Hu
    稀释度
    • ICC

      1:500-1:2000
    • IF

      1:200-1:1000
    背景
    别名
    Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A (CDK4I), Multiple tumor suppressor 1 (MTS-1), p16-INK4a (p16-INK4; p16INK4A), CDKN2A, CDKN2, MTS1
    背景

    p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer.

    细胞定位
    Cytoplasm、Nucleus
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, -20 °C as supplied

    数据库链接
    Accession

    参考图片

    ICC shows positive staining in  HeLa cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    Negative control:ICC shows negative staining in MCF7 cells. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/2000 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    IF shows positive staining in paraffin-embedded Human cervical squamous cell carcinoma. Anti- p16 (Alexa Fluor® 555 Conjugate) antibody was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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    货号:
    S0B1584-1ml
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