首页抗体一抗一抗

p21 Waf1/Cip1 (DCS60) Mouse mAb

1/3

p21 Waf1/Cip1 (DCS60) Mouse mAb

品牌： CST

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

分子量：

反应种属：

Human,Monkey,

展开

产品介绍

产品信息

抗原名称

p21 Waf1Cip1

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant human p21 corresponding to the amino-terminal portion of p21.

宿主

Mouse

简单描述

Monoclonal Antibody for studying p21 Waf1/Cip1. Cited in 365 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody (CST #2946) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:2000
Immunohistochemistry (Paraffin)	1:50

分子量

研究领域

癌症,细胞生物学,

应用

反应种属

Human,Monkey,

目标/特异性

Specificity/Sensitivity

p21 Waf1/Cip1 (DCS60) Mouse mAb detects endogenous levels of total p21 protein. The antibody does not cross-react with other cdk inhibitors.

Species Reactivity:

Human, Monkey

敏感性

Endogenous

背景

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5). 1.Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-34. 2.Cheng, J. et al. (1999) EMBO J. 18, 1571-83. 3.Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-9. 4.Wang, Y. and Prives, C. (1995) Nature 376, 88-91. 5.Sheaff, R.J. et al. (2000) Cell 5, 403-10.

研究领域

癌症,细胞生物学,

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

1026

UniProt ID

P38936

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p21 Waf1/Cip1 (DCS60) Mouse mAb.免疫组织化学检测石蜡包埋人类乳腺癌组织，显示核定位和胞浆定位，使用的抗体为p21 Waf1/Cip1 (DCS60) Mouse mAb。

Western blot analysis of extracts from 293, MCF-7 and COS cells, using p21 Waf1/Cip1 (DCS60) Mouse mAb.Western blot 方法检测293, MCF-7和COS细胞，使用的抗体为 p21 Waf1/Cip1 (DCS60) Mouse mAb。

Western blot analysis of extracts from 293 and Hela cells, transfected with control (-) or p21 Waf1/Cip1 (+) siRNA. p21 Waf1/Cip1 was detected using p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody #2946, p42 MAPK was detected using p42 MAPK (3A7) Mouse Monoclonal Antibody #9107. The p21 Waf1/Cip1 monoclonal antibody confirms silencing of p21 expression, and the p42 monoclonal antibody is used to control for loading and specificity of p21 Waf1/Cip1 siRNA. Western blot方法分析293和Hela细胞提取物。分别用control (-)或 p21 Waf1/Cip1 (+) siRNA转染细胞。使用的抗体为p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody #2946和p42 MAPK (3A7) Mouse Monoclonal Antibody #9107。和对照抗体p42 monoclonal antibody相比，p21 Waf1/Cip1 monoclonal antibody的检测结果证实p21的表达被p21 Waf1/Cip1 siRNA特异性沉默。

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

2946T

一键复制

询价

供应商现货

20ul

100ul

300ul

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价