Ascites Mouse Anti-PARP(C2-10)

PARP

Mouse IgG1

Cow PARP (full-length; thymus derived)

PARP [Poly(ADP-Ribose) Polymerase] is a 113 kDa nuclear chromatin associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular reponse to DNA damage and is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a 113 kDa intact form into 89 kDa and 24 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the C-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis.  This antibody has been reported to recognize an epitope located within the DNA-binding domain of the enzyme.

PARP [Poly(ADP-Ribose) Polymerase] is a 113 kDa nuclear chromatin associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular reponse to DNA damage and is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a 113 kDa intact form into 89 kDa and 24 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the C-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis.  This antibody has been reported to recognize an epitope located within the DNA-binding domain of the enzyme.

Mouse IgG1

C2-10

0.5 mg/ml

Western blot (Routinely Tested) Immunofluorescence (Reported)

Human (QC Testing)

Poly (ADP-Ribose) Polymerase

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 1993; 53(17):3976-3985. (Biology: Western blot). 2. Lamarre D, Talbot B, Leduc Y, Muller S, Poirier G. Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase. Biochem Cell Biol. 1986; 64(4):368-376. (Immunogen). 3. Lamarre D, Talbot B, de Murcia G, et al. Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study. Biochim Biophys Acta. 1988; 950(2):147-160. (Clone-specific: Immunofluorescence). 4. Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996; 10(5):587-597. (Biology). 5. Tewari M, Quan LT, O'Rourke K, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell. 1995; 81(5):801-809. (Biology: Western blot).