Rabbit anti-PARP1 Polyclonal Antibody

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Rabbit anti-PARP1 Polyclonal Antibody

品牌： Absin

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参数

分子量：

Detects a band of approximately 89kD/113kD (Predicted molecular weight: 113kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human, Mouse, Rat

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产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

Affinity purification

宿主

Rabbit

免疫原

A synthetic peptide corresponding to a sequence within amino acids 81-390 of human PARP1 (NP_001609.2).

同种型

IgG

分子量

克隆类别

Polyclonal Antibody

应用

实验应用

WB: 1:1000-1:5000, IF: 1:50-1:200, ICC: 1:50-1:200

反应种属

Human, Mouse, Rat

阳性对照

U-87MG,SKOV3,HeLa,Jurkat,HepG2

背景

别名

PARP1抗体

翻译后修饰

Unmodified

细胞定位

Nucleus,nucleolus

制备和贮存

存储溶液

PBS with 0.01% thimerosal,50% glycerol,pH7.3.

保存方式

Store at -20℃. Avoid freeze/thaw cycles.

参考图片

Western blot analysis of extracts of various cell lines, using PARP1 antibody (abs115182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using PARP1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 antibody at dilution of 1:100 (40x lens).

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货号：

abs115182-50ul

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询价

50ul

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