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Rabbit anti-PARP1 Polyclonal Antibody


品牌: Absin








分子量:
Detects a band of approximately 120kD (Predicted molecular weight: 113kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Detects a band of approximately 120kD (Predicted molecular weight: 113kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
反应种属:
Human;Mouse
Human;Mouse
产品介绍
产品信息
耦联标记
Unlabeled

纯化方式
Affinity purification

宿主
Rabbit

免疫原
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PARP1 (NP_001609.2).

同种型
IgG

分子量
Detects a band of approximately 120kD (Predicted molecular weight: 113kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

克隆类别
Polyclonal Antibody

应用
实验应用
WB: 1:500-1:2000

反应种属
Human;Mouse

阳性对照
Jurkat,293T,BT-474,Mouse thymus

背景
别名
PARP1抗体

翻译后修饰
Unmodified

细胞定位
Nucleus,nucleolus

制备和贮存
存储溶液
PBS with 0.01% thimerosal,50% glycerol,pH7.3.

保存方式
Store at -20℃. Avoid freeze/thaw cycles.
参考图片
Western blot analysis of extracts of various cell lines, using PARP1 antibody (abs134014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 20s.
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