PAXgene Blood ccfDNA Tube (100)

• Single-tube collection, stabilization, transport and storage
• Immediate stabilization of circulating, cell-free DNA (ccfDNA)
• Sample transport and storage at room temperature for up to 10 days
• Standardized preanalytical processing of samples

PAXgene Blood ccfDNA Tubes simplify the collection and processing of whole blood for subsequent purification of circulating, cell-free DNA (ccfDNA). The tube additive stabilizes blood cells (see " PAXgene Blood ccfDNA stabilization helps prevent hemolysis allowing distinct separation of plasma" and " PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions") and blocks apoptosis (see " PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA"). Consequently, using PAXgene Blood ccfDNA Tubes for whole blood collection, storage and transport helps prevent the release of intracellular DNA into plasma (see " PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma"). ccfDNA levels are stable for up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C prior to processing, so blood samples can be transported and stored to process plasma later.
Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes and processed with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, yields high-quality ccfDNA for downstream analytical assays, including but not limited to PCR-based assays and next-generation sequencing (NGS).

ccfDNA purified from plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be used in a wide range of downstream applications, including:

• PCR, including digital, multiplex and quantitative real-time PCR
• Pharmacogenomic studies
• SNP genotyping
• Next-generation sequencing