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PCNA (PC10) Mouse mAb
PCNA (PC10) Mouse mAb

PCNA (PC10) Mouse mAb

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品牌： CST
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    分子量：
    36
    36
    反应种属：
    Human,Mouse,Rat,Monkey,Bovine,Pig,
    Human,Mouse,Rat,Monkey,Bovine,Pig,
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    产品介绍
    产品信息
    抗原名称
    PCNA
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
    宿主
    Mouse
    简单描述
    Monoclonal Antibody for studying PCNA. Cited in 579 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, PCNA (PC10) Mouse Monoclonal Antibody (CST #2586) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:2000
    Immunoprecipitation1:100
    Immunohistochemistry (Paraffin)1:8000 - 1:32000
    Immunofluorescence (Immunocytochemistry)1:1600 - 1:3200
    Flow Cytometry (Fixed/Permeabilized)1:400
    分子量
    36
    研究领域
    癌症,细胞生物学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,Bovine,Pig,
    目标/特异性

    Specificity/Sensitivity

    PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine, Pig

    敏感性
    Endogenous
    背景
    背景
    Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation. 1.Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20. 2.Krishna, T.S. et al. (1994) Cell 79, 1233-43. 3.Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60. 4.Sawey, E.T. et al. (2011) Cancer Cell 19, 347-58.
    研究领域
    癌症,细胞生物学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #85118.
    数据库链接
    Entrez-Gene ID
    5111
    UniProt ID
    P12004

    参考图片

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类结肠癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类乳腺癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。

    Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类非霍奇金淋巴瘤癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。

    Western blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967(lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2).Western blot 方法检测Hela细胞提取物（泳道1）和用PCNA (PC10) Mouse mAb 从同一提取物中免疫沉淀的PCNA（泳道2），使用的抗体为PCNA (PC10) Mouse mAb (上图) 和 Beta-Actin Antibody #4967(下图)。

    Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.Western blot 方法检测人类Hela细胞，鼠SV-T2细胞，牛BAEC细胞及猪PAE细胞提取物，使用的抗体为PCNA (PC10) Mouse mAb。

    Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (green), Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate) (red), and p21 Waf1/Cip1 (12D1) Rabbit mAb (blue).激光共聚焦免疫荧光方法检测NCI-H460细胞，使用的抗体为PCNA (PC10) Mouse mAb ，呈绿色，Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate)，呈红色，及 p21 Waf1/Cip1 (12D1) Rabbit mAb ，呈蓝色。

    Flow cytometric analysis of Jurkat cells using PCNA (PC10) Mouse mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

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    货号：
    2586S
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