Purified Mouse Anti-PCNA(24/PCNA)

Unconjugated

PCNA

Mouse IgG1

Human PCNA aa. 68-230

Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins; 2) synthesis and  degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.

Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins; 2) synthesis and  degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.

Mouse IgG1

24/PCNA

250 µg/ml

Western blot (Routinely Tested) Immunohistochemistry, Immunofluorescence (Tested During Development) Immunoprecipitation (Not Recommended)

Human (QC Testing), Mouse, Rat, Dog (Tested in Development)

Proliferating Cell Nuclear Antigen

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

研发参考(5) 1. Li Y, Dowbenko D, Lasky LA. AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J Biol Chem. 2002; 277(13):11352-11361. (Biology: Western blot). 2. Moore JK, Scheinman RI, Bellgrau D. The identification of a novel T cell activation state controlled by a diabetogenic gene. J Immunol. 2001; 166(1):241-248. (Biology: Western blot). 3. Reilly PT, Wysocka J, Herr W. Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis. Mol Cell Biol. 2002; 22(19):6767-6778. (Biology: Western blot). 4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence). 5. Travali S, Ku DH, Rizzo MG, Ottavio L, Baserga R, Calabretta B. Structure of the human gene for the proliferating cell nuclear antigen. J Biol Chem. 1989; 264(13):7466-7472. (Biology).