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Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb
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Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb

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品牌： CST
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    分子量：
    58 to 68
    58 to 68
    反应种属：
    Human,Mouse,Rat,
    Human,Mouse,Rat,
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    产品介绍
    产品信息
    抗原名称
    PDK1
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser241 of human PDK1.
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying PDK1 (Ser241) phosphate. Cited in 126 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PDK1 (Ser241) (C49H2) Rabbit Monoclonal Antibody (CST #3438) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:50
    分子量
    58 to 68
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    应用
    反应种属
    Human,Mouse,Rat,
    目标/特异性

    Specificity/Sensitivity

    Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241.

    Species Reactivity:

    Human, Mouse, Rat

    敏感性
    Endogenous
    背景
    背景
    Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4). 1.Belham, C. et al. (1999) Curr. Biol. 9, R93-R96. 2.Toker, A. and Newton, A.C. (2000) Cell 103, 185-188. 3.Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448. 4.Casamayor, A. et al. (1999) Biochem J 342 (Pt 2), 287-92.
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    翻译后修饰
    Phosphate
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    5170
    UniProt ID
    O15530

    参考图片

    Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).

    Western blot分析PC3 细胞提取物，未处理组和λ磷酸酶处理组， 所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上), PDK1 Antibody #3062 (中) 和 Akt Antibody #9272 (下)。

    Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

    Western blot分析PC3、 HCT116 wild-type 和 HCT116 PDK1 -/- cells 细胞提取物，所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下). (HCT116 wild-type和HCT116 PDK1 -/- 细胞 由Johns Hopkins University, Baltimore, MD的Dr. Bert Vogelstein友情提供)。

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    货号：
    3438T
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