
产品介绍
产品信息
抗原名称
CD31

来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala451 of mouse CD31 (PECAM-1) protein.

宿主
Rabbit

简单描述
Monoclonal Antibody for studying PECAM-1. Cited in 237 publications. Validated for WB, IHC, IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (D8V9E) XP® Rabbit Monoclonal Antibody (CST #77699) is ready to ship.

商品描述
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
IHC Leica Bond | 1:50 - 1:200 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |

分子量
135

研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,

应用
反应种属
Mouse

目标/特异性
Specificity/Sensitivity
CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb recognizes endogenous levels of total CD31 (PECAM-1) protein.
Species Reactivity:
Mouse

敏感性
Endogenous

背景
背景
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
1.Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
2.Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72.
3.Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24.

研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,
翻译后修饰
Unmodified

制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #92841.
数据库链接
Entrez-Gene ID
5175

UniProt ID
P16284

参考图片
Immunohistochemical analysis of paraffin-embedded A2058 xenograft using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse liver using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse kidney using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.
Western blot analysis of extracts from 32D (CD31/PECAM-1 positive) and 3T3 (CD31/PECAM-1 negative) cells using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
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