Percoll®

无

Percoll® is used in density centrifugation applications for the separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity. Percoll® consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll® is non-toxic, almost chemically inert and does not adhere to membranes. Percoll® is adjustable to physiological ionic strength and pH, and gradients can either be performed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor. Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/ml. Percoll® has also been used in sperm preparation to study the energy requirements of bovine embryos.

suspension

l-谷氨酰胺5%、维生素2%、无机盐3%，蒸馏水90%

Percoll® is low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses