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品牌: BD Pharmingen








实验应用:
Intracellular staining (flow cytometry) (Routinely Tested)
Intracellular staining (flow cytometry) (Routinely Tested)
产品介绍
产品信息
简单描述
BD Phosflow™ Perm Buffer III is intended to be used for the permeabilization of fixed PBMCs or lysed whole blood. PBMCs, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD Phosflow™ Perm Buffer III to allow for an effective intracellular staining of kinases. Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer III can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases). In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers). Not all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer III. For a current listing of compatible cell surface markers for use with BD Phosflow™ Perm Buffer III, investigators are encouraged to visit the following website: http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

商品描述
BD Phosflow™ Perm Buffer III is intended to be used for the permeabilization of fixed PBMCs or lysed whole blood. PBMCs, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD Phosflow™ Perm Buffer III to allow for an effective intracellular staining of kinases. Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer III can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases). In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers). Not all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer III. For a current listing of compatible cell surface markers for use with BD Phosflow™ Perm Buffer III, investigators are encouraged to visit the following website: http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)

制备和贮存
存储溶液
Aqueous buffered solution containing proprietary ingredients.

参考图片
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