Perm Buffer III

BD Phosflow™ Perm Buffer III is intended to be used for the permeabilization of fixed PBMCs or lysed whole blood.  PBMCs, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD Phosflow™ Perm Buffer III to allow for an effective intracellular staining of kinases.  Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer III can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases).  In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers).  Not all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer III.  For a current listing of compatible cell surface markers for use with BD Phosflow™ Perm Buffer III, investigators are encouraged to visit the following website:  http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

BD Phosflow™ Perm Buffer III is intended to be used for the permeabilization of fixed PBMCs or lysed whole blood.  PBMCs, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD Phosflow™ Perm Buffer III to allow for an effective intracellular staining of kinases.  Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer III can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases).  In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers).  Not all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer III.  For a current listing of compatible cell surface markers for use with BD Phosflow™ Perm Buffer III, investigators are encouraged to visit the following website:  http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

Intracellular staining (flow cytometry) (Routinely Tested)

Aqueous buffered solution containing proprietary ingredients.