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PHENOVUE FLUOR 594 - WGA
PHENOVUE FLUOR 594 - WGA

PHENOVUE FLUOR 594 - WGA

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品牌： Revvity
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    产品介绍
    产品信息

    Overview

    WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

    Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

    PhenoVue Fluor 594 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

    Additional product information

    Features
    Numbers of vials per unit5
    Quantity or Volume Per Vial1mg (29.2nmoles)​
    FormLyophilized​
    Storage2-8°C​
    Recommended working concentration5 µg/mL (146nM)
    Maximum excitation wavelength590 nm
    Maximum emission wavelength617 nm
    Common filter setTexas-Red​
    Live cell stainingYes
    Fixed cell stainingYes. See Product Information Sheet for more information
    Equivalent number of microplates36-100 x 96-well microplates
    30-100​ x 384-well microplates
    55-160​ x 1536-well microplates

     

    Specifications

    Color
    Red
    Brand
    PhenoVue™
    Detection Method
    Fluorescence
    Filter
    Texas-Red
    Fluorophore
    PhenoVue™ Fluor 594
    Organelle and Cell Compartment
    Golgi Apparatus
    Product Compatibility
    Live and fixed samples
    Shipping Conditions
    Shipped Ambient
    Type
    Individual Reagent
    Unit Size
    5 vials

    Image gallery

    PhenoVue Fluor 594 - WGA
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red). Imaged on Operetta CLS.
    HeLa cells stained with PhenoVue Fluor 594 - WGA
    PhenoVue Fluor 594 - WGA
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red). Imaged on Operetta CLS.
    HeLa cells stained with PhenoVue Fluor 594 - WGA
    PhenoVue Fluor 594 - WGA
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red). Imaged on Operetta CLS.
    HeLa cells stained with PhenoVue Fluor 594 - WGA
    PhenoVue Fluor 594 - WGA
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
    HeLa fixed (PFA) and permeabilized (0.1 Triton X100) cells stained with PhenoVue Fluor 594 WGA (Plasma Membrane + Golgi Apparatus, red). Imaged on Operetta CLS.
    HeLa cells stained with PhenoVue Fluor 594 - WGA

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    货号：
    CP15941
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