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Ponceau S
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简单描述
Buffer for studying Ponceau Stain in the research area.
商品描述
Product Usage Information
1. Following protein electrotransfer, rinse membrane briefly in ddH2O to remove any detergent that may inhibit staining.2. Incubate membrane in Ponceau S Staining Solution for 5-10 minutes at room temperature.3. Wash membrane in ddH2O until distinct reddish-pink protein bands are visible (1-5 min).4. If desired, mark proteins with waterproof ink or a pencil. Alternatively, a photograph of the stained membrane can also be taken.5. Wash membrane in 1X TBS-T several times for 5 min at room temperature until protein bands are no longer visible.6. Proceed with blocking step of Western blot procedure.
组成成分
丽春红S（酸性红112/3-羟基-4-[2-磺酸基-4-(4-磺酸苯基偶氮)苯基偶氮]-2,7-萘二磺酸四钠）0.1%，乙醇水溶液0.1%，其余为水
背景
背景
Ponceau S is a negatively charged, red colored stain which binds to positively charged amino groups and non-polar regions of proteins. It has a detection limit of around 250 nanograms of protein following SDS-PAGE and electrotransfer to nitrocellulose membranes (1).
1.Salinovich, O. and Montelaro, R.C. (1986) Anal Biochem 156, 341-7.
制备和贮存
保存方式
Store at room temperature. This product is stable for 12 months. Do not dilute or aliquot.
参考图片
Titration of BSA visualized by Ponceau S Staining Solution after polyacrylamide gel electrophoresis and transfer to nitrocellulose membrane.
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危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
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