PNGase F

1/2

PNGase F

品牌： UA BIOSCIENCE

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参数

分子量：

35.7kDa (Reducing)

纯度：

＞95% by SDS-PAGE

产品介绍

产品信息

耦联标记

Unconjugated

氨基酸序列

分子量

35.7kDa (Reducing)

生物活性

40000 U/ml

酶活定义

One unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of carbohydrate from 10μg denatured RNaseB at 37℃ for 1 hour in a 10μL reaction system.

纯度

＞95% by SDS-PAGE

性状

Liquid

表达系统

E.coli

缓冲体系

20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% Glycerol, pH7.5

组合货号

Components	Amount
PNGase F*	40000U/ml
Reaction Buffer (10×)	200mM Tris, PH 7.5 25°C
Denaturing Buffer(10×)	5% SDS、400 mM DTT
NP-40(10×)	10% NP-40 in MilliQ-H2O

* One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl.

标签

His Tag

种属

Elizabethkingia miricola

电泳JSON

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背景

别名

Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F

背景

Peptide: N-glycosidase F (PNGase F) is an asparagine amidase produced by Flavobacterium meningosept-icum that serves as a useful tool in the research on protein N-glycosylation. Recombinant expression in E.coli. The cleavage site of PNGase F is the amide bond between N-acetylglucosamine (GlcNAc) and aspartate residues on the medial side of the glycoprotein, and converts aspartyl to aspartic acid on the enzymolysis protein. This product is often used for complete deglycosylation of antibodies and their associated proteins.

The PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins. The kit includes recombinant Peptide N-Glycosidase F(PNGase F) enzyme, which cleaves N-glycan chains at the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides, and a 10X reaction buffer.

制备和贮存

保存方式

· 12 months from date of receipt, -20 to -70 °C as supplied.
· Please avoid repeated freeze-thaw cycles.

文献

1. Frank Maley and Robert B. Trimble and Anthony L. Tarentino and Thomas H. Plummer Jr. Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases[J]. Analytical Biochemistry, 1989.

参考图片

M marker

1: Rnase B 10μg

2: Rnase B 10μg+0.25U PNGF

3: Rnase B 10μg+0.5U PNGF

4: Rnase B 10μg+1U PNGF

Complement PNGaseF,1µg on SDS-PAGE under reducing and Non-reducing condition. The Purity is greater than 95%.

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货号：

UA070014-15000U

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