PGE2 Assay Kit (1 Kit)

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PGE2 Assay Kit (1 Kit)

品牌： R&D Systems

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Product Summary

The Parameter PGE₂ Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE₂ in cell culture supernates, serum, plasma, and urine.

Recovery

The recovery of PGE₂ spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	84-106
EDTA Plasma (n=4)	93	82-108
Heparin Plasma (n=4)	94	81-111
Serum (n=4)	97	86-114
Urine (n=4)	99	81-111

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PGE₂ were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Multi-species Prostaglandin E2/PGE2 ELISA Standard Curve

Prostaglandin E2 Parameter Assay Kit Summary

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (51 uL), Serum (51 uL), EDTA Plasma (51 uL), Heparin Plasma (51 uL), Urine (51 uL)

Sensitivity

41.4 pg/mL

Assay Range

39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Measures PGE₂ levels in various samples

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

背景

别名

Dinoprostone,PGE2

背景

Background: Prostaglandin E2/PGE2

Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

Alternate Names:

Dinoprostone; PGE2

研究领域

Related Research Areas

制备和贮存

保存方式

Preparation and Storage

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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货号：

KGE004B

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