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EGFR (L858R) Recombinant Rabbit mAb,PBS Only (SDT-421-202)
EGFR (L858R) Recombinant Rabbit mAb,PBS Only (SDT-421-202)
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EGFR (L858R) Recombinant Rabbit mAb,PBS Only (SDT-421-202)

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品牌： STARTER
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    反应种属：
    Hu
    Hu
    来源宿主：
    Rabbit
    Rabbit
    展开
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A
    抗原名称
    EGFR (L858R)
    宿主
    Rabbit
    免疫原
    Synthetic Peptide
    同种型
    IgG
    克隆号
    SDT-421-202
    浓度
    1 mg/ml
    性状
    Liquid
    缓冲体系
    PBS
    产品类型
    Recombinant mAb
    应用
    实验应用
    WB、IHC-P、IP
    反应种属
    Hu
    稀释度

    • ELISA

    • Sandwich ELISA

    • CLIA

    • Lateral Flow

    • Dot Blot

      1:1000

    • WB

      1:1000

    • IP

    • IHC-P

      1:100-1:200

    • ICC

    • IF

    • ICFCM

    • FCM

    • mIHC

    背景
    别名
    Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1
    背景

    EGFR is a 170 kDa transmembrane receptor that induces tyrosine kinase activity affecting cell growth. EGFR mutations can occur in various parts of the gene. In NSCLC, the mutations occur almost exclusively in the exons encoding the kinase domain (exons 18 to 21). They most commonly include the in-frame deletion of exon 19 (del19) and L858R mutation in exon 21.Del19 constitutes 44–51%, and exon 21 L858R mutation constitutes 38–40%.

    细胞定位
    Cell membrane、Endoplasmic reticulum membrane
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, 4 °C as supplied

    数据库链接
    Accession
    P00533

    参考图片

    Dot blot result of EGFR (L858R) Recombinant Rabbit mAb
    Lane1: EGFR(L858R) mutated peptide
    Lane2: EGFR(L858R) mutated peptide
    Lane3: EGFR unmutated peptide
    Primary antibody: EGFR (L858R) Recombinant Rabbit mAb at 1/1000 dilution
    Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

    WB result of EGFR (L858R) Recombinant Rabbit mAb
    Primary antibody: EGFR (L858R) Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: HeLa whole cell lysate 20 µg
    Lane 2: A431 whole cell lysate 20 µg
    Lane 3: A549 whole cell lysate 20 µg
    Lane 4: HCC827 whole cell lysate 20 µg
    Lane 5: H1975 whole cell lysate 20 µg
    Negative control: HeLa whole cell lysate; A431 whole cell lysate; A549 whole cell lysate; HCC827 whole cell lysate
    Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 134 kDa
    Observed MW: 150 kDa

    IHC shows positive staining in paraffin-embedded human lung adenocarcinoma (Mutated case). Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma (un-mutated case). Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    Negative control: IHC shows negative staining in paraffin-embedded human lung. Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    Expression of EGFR (L858R) in tumor tissue

    Expression of EGFR (L858R) in human tissue

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    货号：
    S0B2355P-1mg
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