首页
FGFR2b Recombinant Rabbit mAb (SDT-423-121)
FGFR2b Recombinant Rabbit mAb (SDT-423-121)
1/6

FGFR2b Recombinant Rabbit mAb (SDT-423-121)

分享
品牌： STARTER
pdf 下载产品说明书
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    反应种属：
    Hu
    Hu
    来源宿主：
    Rabbit
    Rabbit
    展开
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A
    抗原名称
    FGFR2b
    宿主
    Rabbit
    免疫原
    Synthetic Peptide
    同种型
    IgG
    克隆号
    SDT-423-121
    浓度
    0.5 mg/ml
    性状
    Liquid
    缓冲体系
    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
    产品类型
    Recombinant mAb
    应用
    实验应用
    WB、IHC-P
    反应种属
    Hu
    稀释度

    • ELISA

    • Sandwich ELISA

    • CLIA

    • Lateral Flow

    • Dot Blot

    • WB

      1:1000

    • IP

    • IHC-P

      1:1000

    • ICC

    • IF

    • ICFCM

    • FCM

    • mIHC

    背景
    别名
    FGFR2IIIb; Fibroblast growth factor receptor 2b; Fibroblast growth factor receptor 2IIIb
    背景

    FGFR2b is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR2b has important roles in embryonic development and tissue repair, especially bone and blood vessels. Recent studies have found that about 30% of patients overexpress FGFR2b protein, which may be used as a new biomarker and research target for gastric cancer.

    细胞定位
    Secreted、Cell membrane
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, -20 °C as supplied

    数据库链接
    Accession
    P21802-3

    参考图片

    WB result of FGFR2b Recombinant Rabbit mAb
    Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: SW620 whole cell lysate 20 µg
    Lane 2: KATO III whole cell lysate 20 µg
    Negative control: SW620 whole cell lysate
    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
    Observed MW: 90~145 kDa

    WB result of FGFR2b Recombinant Rabbit mAb
    Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: recombinant human FGFR2b protein 10 ng (CAT. NO. UA010145)
    Lane 2: recombinant human FGFR2c protein 10 ng (UniProt: P21802-1)
    Lane 3: recombinant human FGFR1 protein 10 ng (CAT. NO. UA020008)
    Lane 4: recombinant human FGFR3 protein 10 ng
    Lane 5: recombinant human FGFR4 protein 10 ng (CAT. NO. UA020009)
    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
    Observed MW: 38~60 kDa

    IHC shows positive staining in paraffin-embedded human gastric cancer (case 1). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows negative staining in paraffin-embedded human gastric cancer (case 2). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    Expression of FGFR2b in tumor tissue.

    Expression of FGFR2b in human tissue.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    S0B2359-1ml
    一键复制
    询价
    优宁维现货
    1ml
    25μl
    100μl
    500μl
    选择数量
    当前规格1件起购 
    配送至
    预计2-5个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价