THUNDER™Phospho-Rb(S780)TR-FRETCellSignalingAssayKit

THUNDER™Phospho-Rb(S780)TR-FRETCellSignalingAssayKit

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品牌: Bioauxilium
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    反应种属:
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    RB
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S780) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    Components
    Eu-phospho-Rb-S780 (250 µL)
    Acc-phospho-Rb-S780 (1 mL)
    Rb control lysate (2 x 200 µL)
    5X Lysis Buffer 1 (50 mL)
    10X TR-FRET Detection buffer (2.5 mL)
    100X Phosphatase Inhibitor Cocktail (2.5 mL)

    Reactivity
    Human

    Inhibition of Phospho-Rb-S780 in HT-29 cells
    Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S780.
    Z'-factor of Phospho-Rb (S780) in HT-29 cells
    The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.
    HT-29 control lysate titration (QC Test)
    Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
    应用
    反应种属
    Human
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    货号:
    KIT-RBS780P-5000
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    询价
    5000points
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