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THUNDER™Phospho-Rb(S780)TR-FRETCellSignalingAssayKit

产品介绍
产品信息
抗原名称
RB

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S780) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-Rb-S780 (250 µL)
Acc-phospho-Rb-S780 (1 mL)
Rb control lysate (2 x 200 µL)
5X Lysis Buffer 1 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human

Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S780.

The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human

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