Target: Mouse
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG, light chain specific
Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Rabbit, Rat, Sheep Immunoglobulins
Conjugate: Unconjugated
Product Category: Anti‑Light Chain-Specific Antibodies for Western Blotting after IP
Clonality: Polyclonal
RRID: AB_2338460

Information

Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

AffiniPure™ Goat Anti-Mouse IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Rb,Rat,Shp Ig)