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rmTRANCE/RANK L (E. (10 ug)
rmTRANCE/RANK L (E. (10 ug)
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rmTRANCE/RANK L (E. (10 ug)

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    Scientific Data

    Bioactivity Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) BioactivityView Larger

    Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 0.5-2 ng/mL.

    SDS-PAGE Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) SDS-PAGEView Larger

    1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    462-TEC

    FormulationLyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    462-TEC/CF

    FormulationLyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.5‑2 ng/mL.
    Source
    E. coli-derived mouse TRANCE/TNFSF11/RANK L protein
    Lys158-Asp316, with an N-terminal Met
    Accession #
    Q3TWY5
    N-terminal Sequence
    Analysis
    Met
    Predicted Molecular Mass
    19 kDa
    背景
    别名
    CD254 antigen,CD254,ODF,OPGL,OPGLOPTB2,Osteoclast differentiation factor,Osteoprotegerin ligand,RANK L,RANKL,RANKLreceptor activator of nuclear factor kappa B ligand,Receptor activator of nuclear factor kappa-B ligand,sOdf,TNF-related activation-induced cytokine,TNFSF11,TRANCE,TRANCEODFhRANKL2,tumor necrosis factor (ligand) superfamily, member 11,tumor necrosis factor ligand superfamily member 11,TNF-related Activation-induced Cytokine
    背景

    Background: TRANCE/TNFSF11/RANK L

    TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

    References
    1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
    2. Anderson, D.M. et al. (1997) Nature 390:175.
    3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
    4. Kong, Y-Y. et al. (1999) Nature 397:315.
    Long Name
    TNF-related Activation-induced Cytokine
    Entrez Gene IDs
    8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
    Alternate Names
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is typically 0.5-2 ng/mL.

    1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

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    货号：
    462-TEC-010
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