BD™ Cytometric Bead Array (CBA) Human TGF-β1 Single Plex Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human TGF-β1 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Recovery: Acid treated and diluted cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. The low recovery suggests that this assay may not be optimal for plasma samples.

Figure 1. Example BD CBA Human Soluble Human TGF-β1 Flex Set standard curve. Data acquired on a BD FACSArray™ bioanalyzer and analyzed using the FCAP Array™ Software (Cat. No. 641488).

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human TGF-β1 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Linearity: Acid treated and diluted cell culture supernatant, serum or EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. The poor linearity suggests that this assay may not be optimal for plasma samples.

Recovery: Acid treated and diluted cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. The low recovery suggests that this assay may not be optimal for plasma samples.