PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control

Lineage

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  This lineage set includes the set of ISCT negative markers as a cocktail and also a corresponding isotype control cocktail. Description of Set Components Vial                                                                                           Contents                                                                Purpose          PE hMSC Negative Cocktail                                              CD34 PE (Clone:581)                                     Cocktail to identify potential contaminants                                                                                           CD11b PE (Clone: ICRF44)                                                                                           CD19 PE (Clone: HIB19)                                                                                           CD45 PE (Clone: HI30)                                                                                           HLA-DR PE (Clone: G46-6) PE hMSC Isotype Control Negative Cocktail          mIgG1, κ PE (Clone: X40)                            Corresponding isotype control for PE hMSC                                                                                           mIgG2a, κ PE (Clone:G155-178)                   Negative Cocktail

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  This lineage set includes the set of ISCT negative markers as a cocktail and also a corresponding isotype control cocktail. Description of Set Components Vial                                                                                           Contents                                                                Purpose          PE hMSC Negative Cocktail                                              CD34 PE (Clone:581)                                     Cocktail to identify potential contaminants                                                                                           CD11b PE (Clone: ICRF44)                                                                                           CD19 PE (Clone: HIB19)                                                                                           CD45 PE (Clone: HI30)                                                                                           HLA-DR PE (Clone: G46-6) PE hMSC Isotype Control Negative Cocktail          mIgG1, κ PE (Clone: X40)                            Corresponding isotype control for PE hMSC                                                                                           mIgG2a, κ PE (Clone:G155-178)                   Negative Cocktail

Flow cytometry (Tested During Development)

Human (QC Testing)

研发参考(5) 1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Biology: Flow cytometry). 2. Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology: Flow cytometry). 3. Horwitz EM, Le Blanc K, Dominici M, et. al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy. 2005; 7(5):393-395. (Biology). 4. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-1301. (Biology: Flow cytometry). 5. Sánchez L, Gutierrez-Aranda I, Ligero G, et al. Enrichment of Human ESC-Derived Multipotent Mesenchymal Stem Cells with Immunosuppressive and Anti-Inflammatory Properties Capable to Protect Against Experimental Inflammatory Bowel Disease. Stem Cells. 2010; 29(2):251-262. (Biology: Cell differentiation).