Fixable Viability Stain 700

BD Horizon™ Fixable Viability Stain 700 (FVS700) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion and covalent binding of the dye to the higher levels of total cellular amines when compared to living cells with impermeable plasma membranes.  Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their FVS700 fluorescence intensities 10-20 fold over that of viable cells. The FVS700-labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence. BD Horizon™ Fixable Viability Stain 700 is excited by the Red laser (with an excitation maximum of 657 nm), and has a fluorescence emission maximum of 700 nm.

BD Horizon™ Fixable Viability Stain 700 (FVS700) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion and covalent binding of the dye to the higher levels of total cellular amines when compared to living cells with impermeable plasma membranes.  Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their FVS700 fluorescence intensities 10-20 fold over that of viable cells. The FVS700-labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence. BD Horizon™ Fixable Viability Stain 700 is excited by the Red laser (with an excitation maximum of 657 nm), and has a fluorescence emission maximum of 700 nm.

Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)

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