BD Pharmingen™ PE Mouse Anti-Ki-67

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BD Pharmingen™ PE Mouse Anti-Ki-67

品牌： BD Pharmingen

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反应种属：

Human (QC Testing), Mouse (Tested in Development), Rat, Rhesus (Reported)

来源宿主：

Mouse IgG1, κ

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产品介绍

产品信息

耦联标记

抗原名称

Ki-67

宿主

Mouse IgG1, κ

免疫原

Human Ki-67

简单描述

The B56 monoclonal antibody specifically binds to the Ki-67 antigen that is expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). During the G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations.

商品描述

B56 The B56 monoclonal antibody specifically binds to the Ki-67 antigen that is expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). During the G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations.

同种型

Mouse IgG1, κ

克隆号

克隆 B56 (RUO)

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing), Mouse (Tested in Development), Rat, Rhesus (Reported)

目标/特异性

Ki-67

背景

别名

MKI67; Antigen identified by monoclonal antibody Ki-67; KIA

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

参考图片

Multicolor flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling human peripheral blood mononuclear cells (PBMC; Top Plots) or proliferating cells from the human MOLT-4 (ATCC CRL-1582) cell line (Bottom Plots) were permeabilized and fixed with 70% ice-cold ethanol. The cells were washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656), stained with either PE Mouse IgG1 Isotype Control (Cat. No. 555749; Left Plots) or PE Mouse Anti-Ki-67 antibody (Cat. No. 567719; Right Plots) and counterstained with DAPI Solution (Cat. No. 564907) to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

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货号：

567720

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