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Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb

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Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb

品牌： CST

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参数

分子量：

79, 86

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

Stat3

来源纯化

使用与小鼠 Stat3 中 Tyr705 周围残基相对应的合成磷酸肽，对动物进行免疫接种来产生单克隆抗体。

宿主

Rabbit

简单描述

Monoclonal Antibody for studying STAT3 (Tyr705) phosphate. Cited in 3613 publications. Validated for WB, WB, IP, IHC, IHC, IF, F, ChIP, ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit Monoclonal Antibody (CST #9145) is ready to ship.

商品描述

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:2000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:100
IHC Leica Bond	1:100 - 1:400
Immunohistochemistry (Paraffin)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:100 - 1:200
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400
Chromatin IP	1:100
Chromatin IP-seq	1:100

分子量

79, 86

内毒素水平

仓鼠 , 牛 , 猪 , 马

研究领域

免疫学和肿瘤学,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Hamster,Bovine,Pig,Horse,

目标/特异性

Specificity/Sensitivity

仅在 Stat3的酪氨酸 705 位点被磷酸化时，Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 方可检测内源水平的 Stat3 。该抗体不会与磷酸-EGFR 或其他 Stat 蛋白的相应磷酸酪氨酸发生交叉反应。

物种反应性：

人, 小鼠, 大鼠, 猴

敏感性

Endogenous

背景

Stat3 转录因子是众多细胞因子和生长因子受体的重要信号转导 (1)，对胎鼠发育必不可少 (2)。研究显示 Stat3 在多种人类肿瘤中持续激活 (3,4) ，并有致癌的潜在可能性 (5)，具有抗凋亡活性 (3)。Stat3 通过 Tyr705 位点的磷酸化而激活，从而诱导二聚化、核转位、DNA 结合 (6,7)。转录激活似乎受到 MAPK 或 mTOR 通路介导的 Ser727 位点磷酸化的调控 (8,9)。Stat3 同工型的表达反应了生物学功能，Stat3α (86 kDa) 和 Stat3β (79 kDa) 相对表达水平，依赖于细胞类型、配体暴露或者细胞发育的成熟阶段 (10) 。值得注意的是，Stat3β 蛋白在羧基末端转录激活结构域缺少丝氨酸磷酸化位点 (8)。 1.Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2.Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. 3.Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. 4.Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. 5.Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6.Darnell, J.E. et al. (1994) Science 264, 1415-21. 7.Ihle, J.N. (1995) Nature 377, 591-4. 8.Wen, Z. et al. (1995) Cell 82, 241-50. 9.Yokogami, K. et al. (2000) Curr Biol 10, 47-50. 10.Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

研究领域

免疫学和肿瘤学,神经科学,

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #73533.

数据库链接

Entrez-Gene ID

6774

UniProt ID

P40763

参考图片

Immunnohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织,图片显示了细胞核的定位。所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin embedded human breast carcinoma, specifically endothelial cells, untreated (left) or lambda phosphatase treated (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织尤其是内皮细胞，未经过处理(左图),经过lambda磷酸酶处理(右图), 所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。

Western blot analysis of extracts from IFN-alpha treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb. Note that the basal phospho-Stat3 in A431 is detected by the antibody.Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb抗体研究IFN-alpha处理的Jurkat 细胞和 HeLa 细胞 (左图), EGF 处理 A431 细胞(右图)。注：A431 细胞中的本底phospho-Stat3 是用抗体检测。

Flow cytometric analysis of HeLa cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.流式细胞仪研究未经处理的HeLa细胞(蓝色)和经IFN-α处理的HeLa细胞(绿色)。所用抗体为Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。

Immunohistochemical analysis of frozen H1650 xenograft using Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb.免疫组织化学染色分析冷冻SKOV-3异种移植物,所用抗体为Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb。

Confocal immunofluorescent analysis of HeLa cells, IFN-alpha treated (left) or untreated (right), labeled with Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (green).共聚焦免疫荧光分析经IFNalpha处理的HeLa(左图)或者未经处理的 HeLa细胞(右图) ,Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (绿色)标记。

Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine, using Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋Apc (min/+)小鼠肠。所用抗体为Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。Hep G2 细胞培养至4 x 106, 并经过夜饥饿或IL-6(100 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 或2 μl Normal Rabbit IgG #2729 抗体。用人源 IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).

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货号：

9145T

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