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品牌: CST








分子量:
17,19,35
17,19,35
反应种属:
Human,Mouse,Rat,Monkey,
Human,Mouse,Rat,Monkey,
产品介绍
产品信息
抗原名称
Caspase-3

来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.

宿主
Rabbit

简单描述
Polyclonal Antibody for studying Casp3. Cited in 4629 publications. Validated for WB, WB, IP, IHC. Highly specific and rigorously validated in-house, Caspase-3 Antibody (CST #9662) is ready to ship.

商品描述
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:1000 |

分子量
17,19,35

内毒素水平
猪

研究领域
癌症,细胞生物学,纤维化,神经科学,

应用
反应种属
Human,Mouse,Rat,Monkey,

目标/特异性
Specificity/Sensitivity
Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).
Species Reactivity:
Human, Mouse, Rat, Monkey

敏感性
Endogenous

背景
背景
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) 是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚 (ADP-ribose) 聚合酶 (PARP) (1)。caspase-3 的激活需要经过其未活化的酶原形式转变为活化的 p17 和 p12 片段的蛋白水解过程。caspase-3 的剪切需要位于 P1 位点的天门冬氨酸残基 (2)。
Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
Nicholson, D.W. et al. (1995) Nature 376, 37-43.

研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
Unmodified

制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
836

UniProt ID
P42574

参考图片
Immunohistochemical staining of paraffin-embedded human tonsil, showing cytoplasmic localization, using Caspase-3 Antibody.
免疫组化分析石蜡包埋的人扁桃体切片,显示caspase-3定位胞浆,抗体采用Caspase-3 Antibody。
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), and NIH/3T3 cells, untreated or staurosporine-treated (1uM, 3hrs), using Caspase-3 Antibody.
Western blot分析未处理或etoposide (25uM, 5hrs)处理的Jurkat 细胞,以及未处理或 staurosporine (1uM, 3hrs)处理的NIH/3T3 细胞,抗体采用Caspase-3 Antibody.
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