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BD Horizon™ BV421 Rat Anti-Mouse F4/80

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BD Horizon™ BV421 Rat Anti-Mouse F4/80

品牌： BD Pharmingen

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反应种属：

Mouse (QC Testing)

来源宿主：

Rat WI, also known as Wistar (outbred) IgG2a, κ

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产品介绍

产品信息

耦联标记

BV421

抗原名称

F4/80

宿主

Rat WI, also known as Wistar (outbred) IgG2a, κ

免疫原

Mouse F4/80 Recombinant Protein

简单描述

The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 is a 160 kDa glycoprotein that belongs to the EGF-TM7 family of seven-transmembrane spanning cell surface molecules. It is expressed on the surface of granulocytes and a wide range of mature tissue macrophages including, Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria macrophages, and Langerhans cells. F4/80 expression has also been reported on subpopulations of dendritic cells. F4/80 expression is heterogeneous and may be increased during inflammatory responses as observed in various mouse models of colitis, diabetes and brain injury. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

商品描述

T45-2342 The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 is a 160 kDa glycoprotein that belongs to the EGF-TM7 family of seven-transmembrane spanning cell surface molecules. It is expressed on the surface of granulocytes and a wide range of mature tissue macrophages including, Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria macrophages, and Langerhans cells. F4/80 expression has also been reported on subpopulations of dendritic cells. F4/80 expression is heterogeneous and may be increased during inflammatory responses as observed in various mouse models of colitis, diabetes and brain injury. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

同种型

Rat WI, also known as Wistar (outbred) IgG2a, κ

克隆号

克隆 T45-2342 (RUO)

浓度

0.2 mg/ml

产品详情

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:BV421

激发源:Violet 405 nm

最多激发源:407 nm

最多发射源:423 nm

应用

实验应用

Flow cytometry (Routinely Tested), Bioimaging (Tested During Development)

反应种属

Mouse (QC Testing)

目标/特异性

F4/80

背景

别名

Gpf480; F480; Emr1; Ly71; DD7A5-7; EGF-TM7; TM7LN3

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(5) 1. Austyn JM., and Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981; 10:805-815. (Biology). 2. Gordon S, Hamann J, Lin HH, Stacey M. F4/80 and the related adhesion-GPCRs. Eur J Immunol. 2011; 41(9):2472-2476. (Biology). 3. Krüger T, Benke D, Eitner F, et al. Identification and functional characterization of dendritic cells in the healthy murine kidney and in experimental glomerulonephritis. J Am Soc Nephrol. 2004; 15(3):613-621. (Biology). 4. Leenen PJ, Radosević K, Voerman JS, et al. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.. J Immunol. 1998; 160(5):2166-73. (Biology). 5. McKnight AJ, Macfarlane AJ, Dri P, Turley L, Willis AC, Gordon S. Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with Homology to the G-protein-linked transmembrane & hormone receptor family. J Biol Chem. 1996; 271:486. (Biology).

数据库链接

Entrez-Gene ID

13733

参考图片

Two-color flow cytometric analysis of mouse F4/80 expression. Panel 1 - Spleen. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with Alexa Fluor® 647 Rat Anti-Mouse CD11b antibody (Cat. No. 557686) and either BD Horizon™ BV421 Rat IgG2a Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse F4/80 antibody (Cat. No. 565411; Right Plot). The two-color flow cytometric contour plot showing the correlated expression of F4/80 (or Ig Isotype control staining) versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Panel 2 - Peritoneal. C57BL/6 mouse peritoneal cells were preincubated with Mouse BD Fc Block™ and stained with APC Rat Anti-Mouse CD117 (Cat. No. 553356/561074) and BV421 Rat Anti-Mouse F4/80 antibodies. The two-color contour plot showing the correlated expression of F4/80 versus CD117 was derived from gated events with the light-scatter characteristics of viable peritoneal cells. Flow cytometric analyses were performed using a BD LSRFortessa™ Cell Analyzer System. Image analysis of F4/80 expression in mouse spleen. Panel 3 - Spleen. A section from a paraformaldehyde-fixed and frozen normal C57BL/6 mouse spleen was stained with BV421 Rat Anti-Mouse F4/80 (pseudo-colored green), Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683; pseudo-colored red), and BD Horizon™ BV480 Anti-CD3 (pseudo-colored aqua) antibodies. The image was captured on a standard Epifluorescence microscope using 392/23nm excitation and 430/24nm emission.

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货号：

565411

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50ug

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