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BD Pharmingen™ PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail, with Isotype Control

品牌： BD Pharmingen

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参数

反应种属：

Mouse (QC Testing)

实验应用：

Flow cytometry (Routinely Tested)

产品介绍

产品信息

抗原名称

Lineage

简单描述

The PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five PerCP-Cy™5.5-conjugated antibodies is designed to label lineage marker-positive cells for exclusion to facilitate the flow cytometric identification of lineage marker-negative hematopoietic progenitors in mouse bone marrow. Components include clone 145-2C11, which recognizes Mouse CD3e; M1/70, which recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76, mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be combined with the PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail, and the PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail, to further characterize hematopoietic progenitor subpopulations.

克隆号

(RUO)

BD化合物表

描述
数量/尺寸
零件号
EntrezGene ID
PerCP-Cy™5.5 Mouse Lineage Antibody Cocktail
100 Tests (1 ea)
51-9006964
N/A
PerCP-Cy™5.5 Mouse Lineage Isotype Control Cocktail
100 Tests (1 ea)
51-9006977
N/A

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

CD3e, CD11b, CD45R, Ly-76, Ly-6G, Ly-6C

文献

研发参考(6) 1. Goodell MA, Rosenzweig M, Kim H, et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med. 1997; 3(12):1337-1345. (Biology). 2. Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL. Identification of a lineage of multipotent hematopoietic progenitors. Development. 1997; 124(10):1929-1939. (Biology). 3. Okada S, Nakauchi H, Nagayoshi K, Nishikawa S, Miura Y, Suda T. In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells. Blood. 1992; 80(12):3044-3050. (Biology). 4. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5. 5. Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. (Biology). 6. Spangrude GJ, Scollay R. A simplified method for enrichment of mouse hematopoietic stem cells. Exp Hematol. 1990; 18(8):920-926. (Biology).

参考图片

Identification of CD34+ and CD34- subpopulations of hematopoietic progenitor cells. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified Rat anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and stained with either FITC Rat IgG2a κ Isotype Control (Cat. No. 554688; data not shown) or FITC Rat anti-Mouse CD34 mAb RAM34 (Cat. No. 553733) and with either PerCP-Cy™ 5.5 Mouse Lineage Isotype Control Cocktail (Left Panel) or PerCP-Cy™ 5.5 Mouse Lineage Antibody Cocktail (Right Panel). Dead cells were excluded from analysis by staining with 7-AAD (7-Amino-Actinomycin D; Cat. No. 559925). Flow cytometry was performed on a BD LSR™II Flow Cytometry System.

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货号：

561317

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