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BD Horizon™ BV421 Streptavidin
BD Horizon™ BV421 Streptavidin
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BD Horizon™ BV421 Streptavidin

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品牌： BD Pharmingen
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    实验应用：
    Flow cytometry (Routinely Tested), Immunocytochemistry, Immunofluorescence (Tested During Development)
    Flow cytometry (Routinely Tested), Immunocytochemistry, Immunofluorescence (Tested During Development)
    产品介绍
    产品信息
    耦联标记
    BV421
    抗原名称
    Streptavidin
    简单描述
    BD Horizon™ BV421 Streptavidin is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry. Streptavidin was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue TM conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue, and BD Horizon V450 cannot be used simultaneously.
    商品描述
    BD Horizon™ BV421 Streptavidin is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry. Streptavidin was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue TM conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue, and BD Horizon V450 cannot be used simultaneously.
    克隆号
    (RUO)
    浓度
    0.1 mg/ml
    产品详情
    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    BV421
    Violet 405 nm
    407 nm
    423 nm
    应用
    实验应用
    Flow cytometry (Routinely Tested), Immunocytochemistry, Immunofluorescence (Tested During Development)
    目标/特异性
    Streptavidin
    制备和贮存
    存储溶液
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    保存方式
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    文献
    文献
    研发参考(2) 1. Diamandis EP, Christopoulos TK. The biotin-(strept)avidin system: principles and applications in biotechnology. Clin Chem. 1991; 37(5):625-636. (Biology). 2. Shapiro HM. Practical flow cytometry, 4th ed.. Hoboken, N.J.: Wiley-Liss; 2003:1-681.

    参考图片

    Flow cytometric analysis of CD45R/B220 expression on mouse lymphocytes using Biotin-conjugated Anti-Mouse CD45R/B220 antibody and BD Horizon™ BV421 Streptavidin. BALB/c mouse splenic leucocytes were stained with Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085/553086) followed by BD Horizon™ BV421 Streptavidin/SAV (Cat. No. 563259; solid line histogram) or with BD Horizon™ BV421 Streptavidin alone (dashed line histogram). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.

    Immunohistofluorescent analysis of CD45R/B220 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and blocked with 5% goat serum and 1% BSA diluted in 1x PBS. The sections were then treated with an Avidin/Biotin Blocking Kit (Vector Laboratories), and stained with Alexa Fluor® 488 Rat Anti-Mouse CD4 antibody (Cat. No. 557667, pseudo-colored blue), Alexa Fluor® 647 Rat Anti-Mouse CD8a antibody (Cat. No. 557682, pseudo-colored red), and Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085). After washing, sections were then stained with BD Horizon™ BV421 Streptavidin secondary reagent (Cat. No. 563259, pseudo-colored green). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

    Flow cytometric analysis of CD45R/B220 expression on mouse lymphocytes.  BALB/c mouse splenic leucocytes were stained with Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085/553086) followed by BD Horizon™ BV421 Streptavidin/SAV (Cat. No. 563259; solid line histogram) or with BD Horizon™ BV421 Streptavidin alone (dashed line histogram). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.

    Immunohistofluorescent analysis of CD45R/B220 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and blocked with 5% goat serum and 1% BSA diluted in 1x PBS. The sections were then treated with an Avidin/Biotin Blocking Kit (Vector Laboratories), and stained with Alexa Fluor® 488 Rat Anti-Mouse CD4 antibody (Cat. No. 557667, pseudo-colored blue), Alexa Fluor® 647 Rat Anti-Mouse CD8a antibody (Cat. No. 557682, pseudo-colored red), and Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085). After washing, sections were then stained with BD Horizon™ BV421 Streptavidin secondary reagent (Cat. No. 563259, pseudo-colored green). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

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    货号：
    563259
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    100ug
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