ProLong®Gold Antifade Reagent with DAPI

品牌： CST

下载产品说明书

用小程序，查商品更便捷

对比

咨询

产品介绍

产品信息

简单描述

Buffer for studying Prolong Gold Anti-Fade Reagent in the research area.

商品描述

Product Usage Information

Ensure product is brought to RT prior to use.Following immunostaining, cover samples with ProLong® Gold Antifade Reagent with DAPI #8961 and coverslip.To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.

背景

ProLong® Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3). 1.Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68. 2.Kapuscinski, J. (1995) Biotech Histochem 70, 220-33. 3.Manzini, G. et al. (1983) Nucleic Acids Res 11, 8861-76.

制备和贮存

保存方式

Store at -20°C. ProLong® Gold Antifade Reagent is stable for 12 months when stored protected from light, at or below -20°C.

参考图片

Epifluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green) and ProLong® Gold Antifade Reagent with DAPI (blue pseudocolor).外荧光分析HeLa细胞，使用COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853（绿色）和ProLong® Gold Antifade Reagent with DAPI（蓝色假色）。

Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong® Gold Antifade Reagent #9071 (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).共聚焦免疫荧光分析COS-7细胞，用甘油（上面）或ProLong® Gold Antifade Reagent #9071（下面）封固，使用的抗体是：β-Actin (8H10D10) Mouse mAb #3700和Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409（红色）(成像0, 3.3,或10分钟)。

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

8961S

一键复制

询价

供应商现货

10ml

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价