

品牌: Rockland








纯度:
This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
来源宿主:
Staphylococcus aureus
Staphylococcus aureus
产品介绍
产品信息
耦联标记
Fluorescein (FITC)

宿主
Staphylococcus aureus

简单描述
Protein A Fluorescein Conjugated - PA00-02

纯度
This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.

浓度
1.0 mg/mL

防腐剂
0.01% (w/v) Sodium Azide

应用
实验应用
Dot Blot, WB

样本类型以及上样量
5.0 mL

背景
别名
ProA, Staphylococcus A protein, Protein A FITC

背景
Protein A is a 56 kDa cell wall protein originated from Staphylococcus aureus. It contains five homologous Ig-binding domains. Each domain is able to bind the Fc fragment of a wide range of mammalian immunoglobulins. Protein A affinity to Fc may vary in dependence of IgG species origins or subclasses. Protein A is often coupled to solid matrixes such as Sepharose™, magnetic particles or latex for IgG purification from ascites, serum or hybridoma culture media. Protein A is often coupled with reporter molecules such as a fluorescent dye, DyLight™, enzymes, biotin, or other detection agents.

制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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